[白喉类毒素在免疫化学和组织培养检测中的研究]
[Study of diphtheria anatoxins in immunochemical and tissue culture assays].
作者信息
Gal'vidis I A, Sviridov V V
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2007 Jul-Aug(4):28-32.
Equine diphtheria antitoxins from different manufacturers were studied. Their immunochemical interaction with diphtheria toxin, toxoid, and antigens of Corynebacterium diphtheriae in ELISA and immunoblotting assays as well as biological activity in CHO cell assay were compared. The discovered differences between antitoxin samples with stated equal activity in IU/ml point to heterogeneity of antigen composition in preparations used for immunization. Mentioned methods allow to standardize antitoxins basing on their biological activity and immunochemical characteristics.
对来自不同制造商的马源白喉抗毒素进行了研究。比较了它们在ELISA和免疫印迹分析中与白喉毒素、类毒素及白喉棒状杆菌抗原的免疫化学相互作用，以及在CHO细胞试验中的生物活性。在以IU/ml表示活性相同的抗毒素样品之间发现的差异表明用于免疫的制剂中抗原组成存在异质性。上述方法能够根据抗毒素的生物活性和免疫化学特性对其进行标准化。
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