Dmitrieva M N, Gruber I M, Gavrilova N A, Titova N G, Iakovleva I V, Sviridov V V
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 1999 Mar-Apr(2):32-5.
Direct correlation between the results of tests for the biological activity of diphtheria toxin, carried out in vivo guinea pigs and in vitro in the microcytotoxicity test in CHO cell culture, has been established, which makes it possible to use the latter as one of the methods for the rapid, reproducible and economic evaluation of diphtheria toxin. The qualitative and quantitative evaluation of diphtheria toxin in the enzyme immunoassay with the use of monoclonal antibodies and in the microcytotoxicity test demonstrates that these two tests, when used for controlling cultivation processes, have essential advantages over the flocculation test as regards their specificity and information content.
已确定在豚鼠体内进行的白喉毒素生物活性测试结果与在CHO细胞培养的微细胞毒性测试中体外进行的测试结果之间存在直接相关性，这使得可以将后者用作快速、可重复且经济地评估白喉毒素的方法之一。使用单克隆抗体的酶免疫测定和微细胞毒性测试对白喉毒素进行定性和定量评估表明，这两种测试在用于控制培养过程时，在特异性和信息含量方面比絮凝试验具有显著优势。
