Jensen Simon Storgård, Yeo Alvin, Dard Michel, Hunziker Ernst, Schenk Robert, Buser Daniel
Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Berne, Berne, Switzerland.
Clin Oral Implants Res. 2007 Dec;18(6):752-60. doi: 10.1111/j.1600-0501.2007.01417.x. Epub 2007 Sep 20.
A novel biphasic calcium phosphate (CaP) granulate consisting of hydroxyapatite (HA) and beta-tricalciumphosphate (TCP) was compared with pure HA and pure TCP and with autograft as positive control.
Four standardized bone defects were prepared in both mandibular angles of 16 minipigs and grafted with autogenous bone chips, HA, HA/TCP (60% : 40%), or TCP. Histologic and histomorphometric analysis of bone formation and graft degradation followed healing periods of 2, 4, 8, and 24 weeks.
2 weeks: more bone formation in defects filled with autograft than with the three CaP materials (P<0.05). 4 weeks: bone formation differed significantly (P<0.05) between all four materials (autograft>TCP>HA/TCP>HA). 8 weeks: more bone formation in defects with autograft and TCP than with HA/TCP (P<0.05), and HA/TCP had more bone formation than HA (P<0.05). 24 weeks: no difference in bone formation between the groups. Autograft and TCP resorbed quickly and almost completely over 8 weeks, whereas HA/TCP and HA showed limited degradation over 24 weeks.
All defects healed with mature lamellar bone and intimate contact between bone and the remaining graft material. The rate of bone formation corresponded to the content of TCP in the CaP materials.
将一种由羟基磷灰石（HA）和β-磷酸三钙（TCP）组成的新型双相磷酸钙（CaP）颗粒与纯HA、纯TCP以及作为阳性对照的自体骨进行比较。
在16只小型猪的双侧下颌角制备4个标准化骨缺损，并分别植入自体骨碎片、HA、HA/TCP（60% : 40%）或TCP。在2、4、8和24周的愈合期后，对骨形成和移植物降解进行组织学和组织形态计量学分析。
2周时：自体骨填充的缺损处骨形成多于三种CaP材料填充的缺损处（P<0.05）。4周时：四种材料之间的骨形成有显著差异（P<0.05）（自体骨>TCP>HA/TCP>HA）。8周时：自体骨和TCP填充的缺损处骨形成多于HA/TCP填充的缺损处（P<0.05），且HA/TCP的骨形成多于HA（P<0.05）。24周时：各组之间骨形成无差异。自体骨和TCP在8周内迅速吸收且几乎完全吸收，而HA/TCP和HA在24周内降解有限。
所有缺损均愈合为成熟的板层骨，且骨与剩余移植物材料紧密接触。骨形成速率与CaP材料中TCP的含量相对应。
