评估Panbio登革病毒非结构蛋白1抗原检测和免疫球蛋白M抗体酶联免疫吸附测定法在老挝急性登革热感染诊断中的应用。
Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos.
作者信息
Blacksell Stuart D, Mammen Mammen P, Thongpaseuth Soulignasack, Gibbons Robert V, Jarman Richard G, Jenjaroen Kemajittra, Nisalak Ananda, Phetsouvanh Rattanaphone, Newton Paul N, Day Nicholas P J
机构信息
Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
出版信息
Diagn Microbiol Infect Dis. 2008 Jan;60(1):43-9. doi: 10.1016/j.diagmicrobio.2007.07.011. Epub 2007 Sep 24.
We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53-73) on admission samples but was much less sensitive (5%; 95% CI, 1-10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35-55) but was more sensitive on convalescent samples (58%; 95% CI, 48-68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71-87), convalescent samples (63%; 95% CI, 53-73), and all samples (71%; 95% CI, 65-78).
我们评估了两种用于诊断登革热感染的商用酶联免疫吸附测定（ELISA）；一种是检测免疫球蛋白M（IgM）抗体的血清学检测，另一种是基于检测登革热病毒非结构1（NS1）抗原。使用配对血清的金标准参考血清学方法，41%（92例患者中的38例）被确诊为登革热，其中4例（11%）为急性初次感染，33例（87%）为急性二次感染（1例状态不确定）。NS1-ELISA在入院样本上的敏感性为63%（95%置信区间[CI]，53 - 73），但在恢复期样本上敏感性低得多（5%；95% CI，1 - 10）。IgM捕获ELISA与NS1-ELISA相比，入院样本的敏感性较低但在统计学上相当（45%；95% CI，35 - 55），但在恢复期样本上更敏感（58%；95% CI，48 - 68）。NS1和IgM捕获ELISA的结果使用逻辑或运算符进行合并，提高了入院样本（79%；95% CI，71 - 87）、恢复期样本（63%；95% CI，53 - 73）和所有样本（71%；95% CI，65 - 78）的敏感性。
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