氧糖剥夺在体外抑制神经祖细胞的生长和ERK磷酸化。
Oxygen glucose deprivation inhibits the growth and ERK phosphorylation of neural progenitor cells in vitro.
作者信息
Kalluri Haviryaji S G, Eickstaedt Joshua, Dempsey Robert J
机构信息
Department of Neurological Surgery, University of Wisconsin, Madison, WI 53792, USA.
出版信息
Neurosci Lett. 2007 Oct 22;426(3):145-8. doi: 10.1016/j.neulet.2007.08.047. Epub 2007 Aug 25.
Abstract
The neurogenic regions such as subventricular zone of the lateral ventricles could become ischemic in some clinical situations due to the blockage of blood vessels by blood clots. Hence the aim of this study is to investigate the effects of OGD on the growth of neural progenitor cells and the phosphorylation of ERK, which plays an important role in the growth of these cells. Oxygen glucose deprivation (OGD) for 4h decreased the growth of neural progenitor cells in vitro and also decreased the phosphorylation of extracellular signal regulated kinase (ERK). Inhibition of the ERK pathway for 4h using U0126 (10 microM) also decreased the growth of progenitor cells. These data suggest that a decline in the phospho-ERK content might decrease the growth of progenitor cells following OGD.
摘要
在某些临床情况下，由于血凝块堵塞血管，侧脑室室管膜下区等神经源性区域可能会出现局部缺血。因此，本研究的目的是探讨氧糖剥夺（OGD）对神经祖细胞生长及细胞外信号调节激酶（ERK）磷酸化的影响，ERK在这些细胞的生长中起着重要作用。体外进行4小时的氧糖剥夺（OGD）可降低神经祖细胞的生长，并减少细胞外信号调节激酶（ERK）的磷酸化。使用U0126（10微摩尔）抑制ERK通路4小时也会降低祖细胞的生长。这些数据表明，OGD后磷酸化ERK含量的下降可能会降低祖细胞的生长。
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