Dahms H, Hagemann G, Hlinak A
Institut für Virologie und Geflügelkrankheiten Veterinärmedizinische Fakultät Humboldt-Universität zu Berlin.
Zentralbl Veterinarmed B. 1991 Oct;38(8):581-8.
Different serological methods were used in determining the valence of red murrain immune sera. By using a grow test it is possible to accomplish an immunological differentiation of the immune sera. The used ELISA was tested in comparison to the conventional mouse protective test (PRIGGE, 1950) with regard to its valuation of immune sera. Both procedures have been tested on a possible correlation. In almost all cases a correlation between high titres in ELISA and a mouse protective value over 100 I.E. could be proven. The statistical processing of the test results proved an assumed correlation of both procedures. But it is necessary to carry out further research in order to be able to recommend this procedure as an alternative for the mouse protection test.
采用不同的血清学方法测定猪瘟免疫血清的效价。通过生长试验可以实现免疫血清的免疫分化。将所使用的酶联免疫吸附测定(ELISA)与传统的小鼠保护试验(PRIGGE,1950年)在免疫血清评估方面进行了比较测试。对这两种方法的可能相关性进行了测试。在几乎所有情况下,ELISA中的高滴度与超过100个国际单位的小鼠保护值之间的相关性都可以得到证实。对测试结果的统计处理证明了这两种方法之间存在假设的相关性。但为了能够推荐该方法作为小鼠保护试验的替代方法,有必要进行进一步的研究。