Translation of double-stranded RNA segments 1 and 2 of Chuzan virus in rabbit reticulocyte lysates.
作者信息
Yoshida T, Harasawa R, Goto Y
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
出版信息
Zentralbl Veterinarmed B. 1991 Oct;38(8):630-4. doi: 10.1111/j.1439-0450.1991.tb00920.x.
Abstract
Coding assignments of genome segments 1 and 2 of Chuzan virus strain K-47 were studied in vitro by using rabbit reticulocyte lysates. The double-stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)-polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [35S]methionine were resolved by SDS-polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.
摘要
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