Kool Jeroen, Reubsaet Léon, Wesseldijk Feikje, Maravilha Raquel T, Pinkse Martijn W, D'Santos Clive S, van Hilten Jacobus J, Zijlstra Freek J, Heck Albert J R
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Proteomics. 2007 Oct;7(20):3638-50. doi: 10.1002/pmic.200600938.
Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.
早期诊断对于有效的疾病管理至关重要。检测皮肤局部存在的生物标志物可能有助于促进诊断过程。因此，分析代表皮肤间质液的抽吸水疱液蛋白质组，是寻找参与特定疾病生物学途径的潜在生物标志物的理想第一步。在此，我们描述了对抽吸水疱液蛋白质组作为生物标志物蛋白质潜在体液的全面分析。将抽吸水疱液蛋白质组与使用相同方案分析的血清蛋白质组进行比较。通过使用允许假阳性鉴定率低于1%的严格标准，我们能够在相同的实验条件和起始材料量下，在抽吸水疱液中检测到401种蛋白质，在血清中检测到240种蛋白质。作为我们分析的主要结果，我们构建了一份有偏见的列表，列出了34种蛋白质，与血清相比，这些蛋白质在抽吸水疱液中相对高度且独特地被检测到，具有作为生物标志物的既定和推定特征。我们得出结论，对于涉及皮肤的疾病，抽吸水疱液可能潜在地作为一种良好的替代生物标志物体液。
