Nicolaissen B, Eidal K, Haaskjold E, Harsem T, Näss O
Department of Ophthalmology and Pathology, Ulleval Hospital, Oslo, Norway.
Acta Ophthalmol (Copenh). 1991 Dec;69(6):723-30. doi: 10.1111/j.1755-3768.1991.tb02050.x.
The epithelium was removed from human corneas, and samples of conjunctival tissue were cultured as explants on the denuded corneal surface for 1 and 2 weeks. Cells migrating from the conjunctival explants onto the corneal surface produced a multilayer where cells on the surface generally showed a flattened appearance. The apical membrane of these cells demonstrated villi as well as microplicae. Surface projections were also detected on cells in the deeper layers of the epithelium. Neighbouring cells were connected by junctional complexes. After 2 weeks, however, a lack of intercellular junctions in some areas resulted in the formation of intraepithelial cystoid spaces. Basal cells were connected to the underlying basement membrane by hemidesmosomes. Although transdifferentiation of the cells into a corneal epithelium was not observed within the 2 weeks, the present system provides a tool for studies on factors affecting reepithelialization of corneal epithelial defects by conjunctival cells.
从人角膜上移除上皮组织，将结膜组织样本作为外植体在裸露的角膜表面培养1周和2周。从结膜外植体迁移到角膜表面的细胞形成了多层结构，表面的细胞通常呈现扁平外观。这些细胞的顶端膜显示出绒毛以及微褶。在上皮更深层的细胞上也检测到了表面突起。相邻细胞通过连接复合体相连。然而，2周后，一些区域细胞间连接的缺失导致了上皮内囊样间隙的形成。基底细胞通过半桥粒与下方的基底膜相连。尽管在2周内未观察到细胞转分化为角膜上皮，但本系统为研究影响结膜细胞对角膜上皮缺损再上皮化的因素提供了一个工具。
