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2
RRP22 is a farnesylated, nucleolar, Ras-related protein with tumor suppressor potential.RRP22是一种法尼基化的、位于核仁的、具有肿瘤抑制潜力的Ras相关蛋白。
Cancer Res. 2005 Apr 15;65(8):3117-25. doi: 10.1158/0008-5472.CAN-04-0749.
3
Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells.草酸诱导的AMBP基因及其在肾小管上皮细胞中的调控机制。
Biochem J. 2005 May 1;387(Pt 3):609-16. doi: 10.1042/BJ20041465.
4
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Urol Res. 2004 Oct;32(5):357-61. doi: 10.1007/s00240-004-0430-x. Epub 2004 Sep 7.
5
Expression of nuclear pore complex oxalate binding protein p62 in experimental hyperoxaluria.核孔复合物草酸结合蛋白p62在实验性高草酸尿症中的表达
Nephron Exp Nephrol. 2004;97(3):e106-14. doi: 10.1159/000078644.
6
Modulatory effect of the 23-kD calcium oxalate monohydrate binding protein on calcium oxalate stone formation during oxalate stress.23-kD 一水合草酸钙结合蛋白在草酸应激期间对草酸钙结石形成的调节作用
Nephron Physiol. 2004;97(1):p23-30. doi: 10.1159/000077599.
7
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
8
Oxalate binding proteins in calcium oxalate nephrolithiasis.草酸钙肾结石中的草酸结合蛋白
Urol Res. 2003 Aug;31(4):242-56. doi: 10.1007/s00240-003-0316-3. Epub 2003 Jul 11.
9
The role of osteopontin on calcium oxalate crystal formation.骨桥蛋白在草酸钙晶体形成中的作用。
Eur Urol. 2003 May;43(5):564-71. doi: 10.1016/s0302-2838(03)00088-5.
10
Effect of experimental hyperoxaluria on renal calcium oxalate monohydrate binding proteins in the rat.实验性高草酸尿症对大鼠肾草酸钙一水合物结合蛋白的影响。
BJU Int. 2001 Jan;87(1):110-6. doi: 10.1046/j.1464-410x.2001.00972.x.

由45 kDa一水合草酸钙结合蛋白介导的转录抑制

Transcriptional repression mediated by 45-kDa calcium oxalate monohydrate binding protein.

作者信息

Kandaswamy Veena Coothan, Asokan Devarajan, Kalaiselvi Periandavan, Varalakshmi Palaninathan

机构信息

Department of Medical Biochemistry, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, India.

出版信息

Clin Exp Nephrol. 2007 Sep;11(3):196-201. doi: 10.1007/s10157-007-0477-4. Epub 2007 Sep 28.

DOI:10.1007/s10157-007-0477-4
PMID:17891345
Abstract

BACKGROUND

This study was done to investigate the DNA binding ability of a diagnostic biomarker, 45-kDa calcium oxalate monohydrate (COM) binding protein, isolated from human kidney and its effect on transcription.

METHODS

The 45-kDa COM binding protein was isolated and purified from human kidney. The subcellular localization of the protein and the amino acid composition of the protein were analyzed. Oxalate-binding activity in the presence or absence of DNA was determined. The possibility of forming DNA-protein adducts was checked by diethylaminoethyl (DEAE)-Sephadex column chromatography. The effect of the protein on in vitro transcription was also studied.

RESULTS

The isolated 45-kDa protein was found to be basic in nature and its AACompIdent analysis showed it to be related to known transcription factors. The protein was found to be present in kidney cytosol and nucleus. The decreased oxalate binding activity in the presence of the DNA, and the shift in the DEAE-Sephadex elution profile established the DNA-binding ability of the protein. The in vitro transcription assay demonstrated the repression effect of the protein on gene expression during hyperoxaluria.

CONCLUSIONS

Transcriptional repression by the 45-kDa COM binding protein in an in vitro transcription assay system was reduced in the presence of oxalate. Hence, altered expression of certain genes involved in the prognosis of urolithiasis might be mediated by this 45-kDa protein.

摘要

背景

本研究旨在调查从人肾中分离出的一种诊断生物标志物——45 kDa 一水合草酸钙（COM）结合蛋白的 DNA 结合能力及其对转录的影响。

方法

从人肾中分离并纯化 45 kDa COM 结合蛋白。分析该蛋白的亚细胞定位和氨基酸组成。测定有无 DNA 存在时的草酸结合活性。通过二乙氨基乙基（DEAE）-葡聚糖凝胶柱色谱法检查形成 DNA-蛋白质加合物的可能性。还研究了该蛋白对体外转录的影响。

结果

分离出的 45 kDa 蛋白本质上呈碱性，其 AACompIdent 分析表明它与已知转录因子有关。发现该蛋白存在于肾细胞溶质和细胞核中。在有 DNA 存在时草酸结合活性降低，以及 DEAE-葡聚糖凝胶洗脱图谱的变化证实了该蛋白的 DNA 结合能力。体外转录试验证明该蛋白在高草酸尿症期间对基因表达有抑制作用。

结论

在体外转录试验系统中，45 kDa COM 结合蛋白的转录抑制作用在有草酸盐存在时降低。因此，某些参与尿路结石预后的基因表达改变可能由这种 45 kDa 蛋白介导。