地衣芽孢杆菌BlaPβ-内酰胺酶作为研究蛋白质片段插入的模型蛋白支架。
The Bacillus licheniformis BlaP beta-lactamase as a model protein scaffold to study the insertion of protein fragments.
作者信息
Vandevenne Marylène, Filee Patrice, Scarafone Natacha, Cloes Benoît, Gaspard Gilles, Yilmaz Nursel, Dumoulin Mireille, François Jean-Marie, Frère Jean-Marie, Galleni Moreno
机构信息
Macromolécules Biologiques, Centre d'Ingénierie des Protéines, Université de Liège, Liège, Belgium.
出版信息
Protein Sci. 2007 Oct;16(10):2260-71. doi: 10.1110/ps.072912407.
Abstract
Using genetic engineering technologies, the chitin-binding domain (ChBD) of the human macrophage chitotriosidase has been inserted into the host protein BlaP, a class A beta-lactamase produced by Bacillus licheniformis. The product of this construction behaved as a soluble chimeric protein that conserves both the capacity to bind chitin and to hydrolyze beta-lactam moiety. Here we describe the biochemical and biophysical properties of this protein (BlaPChBD). This work contributes to a better understanding of the reciprocal structural and functional effects of the insertion on the host protein scaffold and the heterologous structured protein fragments. The use of BlaP as a protein carrier represents an efficient approach to the functional study of heterologous protein fragments.
摘要
利用基因工程技术，已将人类巨噬细胞几丁质酶的几丁质结合结构域（ChBD）插入宿主蛋白BlaP中，BlaP是地衣芽孢杆菌产生的A类β-内酰胺酶。这种构建产物表现为一种可溶性嵌合蛋白，既保留了结合几丁质的能力，又保留了水解β-内酰胺部分的能力。在此，我们描述了这种蛋白质（BlaPChBD）的生化和生物物理特性。这项工作有助于更好地理解插入对宿主蛋白支架和异源结构化蛋白片段的相互结构和功能影响。使用BlaP作为蛋白质载体代表了一种研究异源蛋白片段功能的有效方法。
相似文献
1
The Bacillus licheniformis BlaP beta-lactamase as a model protein scaffold to study the insertion of protein fragments.
Protein Sci. 2007 Oct;16(10):2260-71. doi: 10.1110/ps.072912407.
2
Rapid and easy development of versatile tools to study protein/ligand interactions.
Protein Eng Des Sel. 2008 Jul;21(7):443-51. doi: 10.1093/protein/gzn021. Epub 2008 May 2.
3
Effects of monopropanediamino-β-cyclodextrin on the denaturation process of the hybrid protein BlaPChBD.
Biochim Biophys Acta. 2011 Sep;1814(9):1146-53. doi: 10.1016/j.bbapap.2011.05.007. Epub 2011 May 15.
4
Crystal structure of the sensor domain of the BlaR penicillin receptor from Bacillus licheniformis.
Biochemistry. 2003 Nov 11;42(44):12835-43. doi: 10.1021/bi034976a.
5
Chitin-binding domain based immobilization of D-hydantoinase.
J Biotechnol. 2005 May 25;117(3):267-75. doi: 10.1016/j.jbiotec.2005.02.001. Epub 2005 Mar 25.
6
Molecular and biochemical characterization of the chromosome-encoded class A beta-lactamase BCL-1 from Bacillus clausii.
Antimicrob Agents Chemother. 2007 Nov;51(11):4009-14. doi: 10.1128/AAC.00537-07. Epub 2007 Sep 10.
7
Characterization of Nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains.
Nucleic Acids Symp Ser (Oxf). 2007(51):459-60. doi: 10.1093/nass/nrm230.
8
Amyloid-like fibril formation by polyQ proteins: a critical balance between the polyQ length and the constraints imposed by the host protein.
PLoS One. 2012;7(3):e31253. doi: 10.1371/journal.pone.0031253. Epub 2012 Mar 9.
9
Stability of a guest protein depends on stability of a host protein in insertional fusion.
Biotechnol Bioeng. 2011 May;108(5):1011-20. doi: 10.1002/bit.23039. Epub 2011 Feb 19.
10
Enzymatic functionalization of a nanobody using protein insertion technology.
Protein Eng Des Sel. 2015 Oct;28(10):451-60. doi: 10.1093/protein/gzv020. Epub 2015 Apr 6.
引用本文的文献
1
: From Taxonomy to Biotechnological and Industrial Perspectives.
Microorganisms. 2022 Nov 28;10(12):2355. doi: 10.3390/microorganisms10122355.
2
The Right-Handed Parallel β-Helix Topology of Pectin Methylesterase Is Intimately Associated with Both Sequential Folding and Resistance to High Pressure.
Biomolecules. 2021 Jul 22;11(8):1083. doi: 10.3390/biom11081083.
3
Reflections on professor Sir Christopher M. Dobson (1949-2019).
Biophys Rev. 2020 Feb;12(1):13-18. doi: 10.1007/s12551-020-00612-9. Epub 2020 Jan 24.
4
Equilibrium partially folded states of B. licheniformis-lactamase.
Eur Biophys J. 2019 May;48(4):341-348. doi: 10.1007/s00249-019-01361-8. Epub 2019 Mar 30.
5
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions.
J Vis Exp. 2018 Feb 1(132):55414. doi: 10.3791/55414.
6
In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model.
J Negat Results Biomed. 2017 Aug 22;16(1):14. doi: 10.1186/s12952-017-0080-5.
7
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.
BMC Biotechnol. 2017 Jul 14;17(1):62. doi: 10.1186/s12896-017-0382-1.
8
Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who's Leading HCHT's Biological Function.
Sci Rep. 2017 Jun 5;7(1):2768. doi: 10.1038/s41598-017-02382-z.
9
Class A β-lactamases as versatile scaffolds to create hybrid enzymes: applications from basic research to medicine.
Biomed Res Int. 2013;2013:827621. doi: 10.1155/2013/827621. Epub 2013 Aug 28.
10
Amyloid-like fibril formation by polyQ proteins: a critical balance between the polyQ length and the constraints imposed by the host protein.
PLoS One. 2012;7(3):e31253. doi: 10.1371/journal.pone.0031253. Epub 2012 Mar 9.
本文引用的文献
1
Active TEM-1 beta-lactamase mutants with random peptides inserted in three contiguous surface loops.
Protein Sci. 2006 Oct;15(10):2323-34. doi: 10.1110/ps.062303606. Epub 2006 Sep 8.
2
Folding and stability of chimeric immunofusion VL-barstar.
Biochemistry (Mosc). 2004 Sep;69(9):939-48. doi: 10.1023/b:biry.0000043536.94292.7d.
3
Carbohydrate binding specificity of the recombinant chitin-binding domain of human macrophage chitinase.
Biosci Biotechnol Biochem. 2003 Nov;67(11):2402-7. doi: 10.1271/bbb.67.2402.
4
A secondary drug resistance mutation of TEM-1 beta-lactamase that suppresses misfolding and aggregation.
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):283-8. doi: 10.1073/pnas.98.1.283.
5
Export and folding of signal-sequenceless Bacillus licheniformis beta-lactamase in Escherichia coli.
Eur J Biochem. 2000 Jun;267(12):3836-47. doi: 10.1046/j.1432-1327.2000.01422.x.
6
Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.
J Bacteriol. 2000 Jun;182(11):3045-54. doi: 10.1128/JB.182.11.3045-3054.2000.
7
Functionally accepted insertions of proteins within protein domains.
J Biol Chem. 2000 Jun 9;275(23):17428-33. doi: 10.1074/jbc.M000666200.
8
Structural and functional definition of the human chitinase chitin-binding domain.
J Biol Chem. 2000 Jan 7;275(1):514-20. doi: 10.1074/jbc.275.1.514.
9
Thermodynamic stability of a cold-active alpha-amylase from the Antarctic bacterium Alteromonas haloplanctis.
Biochemistry. 1999 Apr 6;38(14):4613-9. doi: 10.1021/bi982650+.
10
Identification of viral mutants by mass spectrometry.
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8596-601. doi: 10.1073/pnas.95.15.8596.
Zotero 插件