克劳氏芽孢杆菌染色体编码的A类β-内酰胺酶BCL-1的分子和生化特性
Molecular and biochemical characterization of the chromosome-encoded class A beta-lactamase BCL-1 from Bacillus clausii.
作者信息
Girlich Delphine, Leclercq Roland, Naas Thierry, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 K. Bicêtre Cedex, France.
出版信息
Antimicrob Agents Chemother. 2007 Nov;51(11):4009-14. doi: 10.1128/AAC.00537-07. Epub 2007 Sep 10.
Abstract
A chromosomal beta-lactamase gene from Bacillus clausii NR, which is used as a probiotic, was cloned and expressed in Escherichia coli. It encodes a clavulanic acid-susceptible Ambler class A beta-lactamase, BCL-1, with a pI of 5.5 and a molecular mass of ca. 32 kDa. It shares 91% and 62% amino acid identity with the chromosomally encoded PenP penicillinases from B. clausii KSM-K16 and Bacillus licheniformis, respectively. The hydrolytic profile of this beta-lactamase includes penicillins, narrow-spectrum cephalosporins, and cefpirome. This chromosome-encoded enzyme was inducible in B. clausii, and its gene is likely related to upstream-located regulatory genes that share significant identity with those reported to be upstream of the penicillinase gene of B. licheniformis. The bla(BCL-1) gene was located next to the known chromosomal aadD2 gene and the erm34 gene, which encode resistance to aminoglycosides and macrolides, respectively. Similar genes were found in a collection of B. clausii reference strains.
摘要
来自用作益生菌的克劳氏芽孢杆菌NR的一个染色体β-内酰胺酶基因被克隆并在大肠杆菌中表达。它编码一种对克拉维酸敏感的安布勒A类β-内酰胺酶BCL-1，其等电点为5.5，分子量约为32 kDa。它与克劳氏芽孢杆菌KSM-K16和地衣芽孢杆菌染色体编码的PenP青霉素酶分别具有91%和62%的氨基酸同一性。这种β-内酰胺酶的水解谱包括青霉素、窄谱头孢菌素和头孢匹罗。这种染色体编码的酶在克劳氏芽孢杆菌中是可诱导的，其基因可能与上游的调控基因相关，这些调控基因与报道的地衣芽孢杆菌青霉素酶基因上游的调控基因具有显著同一性。bla(BCL-1)基因位于已知的染色体aadD2基因和erm34基因旁边，这两个基因分别编码对氨基糖苷类和大环内酯类的抗性。在一组克劳氏芽孢杆菌参考菌株中发现了类似的基因。
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Biol Pharm Bull. 2007 Mar;30(3):569-74. doi: 10.1248/bpb.30.569.
2
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J Appl Microbiol. 2006 Dec;101(6):1208-15. doi: 10.1111/j.1365-2672.2006.03042.x.
3
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J Med Food. 2006 Fall;9(3):321-7. doi: 10.1089/jmf.2006.9.321.
4
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7
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