原发性开角型青光眼和假性剥脱性青光眼患者房水中的IgG抗体模式。

IgG antibody patterns in aqueous humor of patients with primary open angle glaucoma and pseudoexfoliation glaucoma.

作者信息

Joachim Stephanie C, Wuenschig Diana, Pfeiffer Norbert, Grus Franz H

机构信息

Experimental Ophthalmology, Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany.

出版信息

Mol Vis. 2007 Sep 5;13:1573-9.

PMID:17893658

Abstract

PURPOSE

There has been growing body of evidence indicating an immunological component in the pathogenesis of glaucoma. Several studies found specific antibodies in sera of glaucoma patients. Recently, we detected antibody profiles in aqueous humor of patients with normal tension glaucoma that showed significant differences when compared to control subjects. The aim of this study was to compare the IgG antibody patterns against retinal antigens in aqueous humor of patients with primary open angle glaucoma, pseudoexfoliation glaucoma, and control subjects then to identify some of the important biomarkers of this study.

METHODS

Aqueous humor of 44 patients was analyzed: 15 patients with primary open angle glaucoma (POAG), 14 patients with pseudoexfoliation glaucoma (PEX), and 15 control subjects (CO). The aqueous was tested against western blots of bovine retinal antigens. The IgG antibody patterns were analyzed by multivariate statistical techniques. Some of the important biomarkers were identified via mass spectrometry (Maldi-TOFTOF).

RESULTS

All patients showed complex patterns of IgG antibodies against retinal antigens. The discriminant analysis revealed a statistically significant difference between the antibody profiles of the POAG and the CO group (p=0.00018). There was also a statistically significant difference between the antibody profiles of the PEX and the CO group (p=0.0013). Not only were up-regulations in the glaucoma groups observed, compared to controls, but also down-regulations. There was no significant difference between the antibody patterns of the POAG and PEX group. The identified biomarkers included heat shock protein 27, alpha-enolase, actin, and GAPDH.

CONCLUSIONS

We could show significant differences between the IgG antibody profiles of the glaucoma groups (PEX and POAG) and controls. The analysis of intraocular antibodies could provide further hints for autoimmune involvement in glaucoma.

摘要

目的

越来越多的证据表明免疫因素在青光眼发病机制中起作用。多项研究在青光眼患者血清中发现了特异性抗体。最近，我们检测了正常眼压性青光眼患者房水中的抗体谱，与对照组相比有显著差异。本研究旨在比较原发性开角型青光眼、剥脱性青光眼患者房水中针对视网膜抗原的IgG抗体模式与对照组，进而确定本研究的一些重要生物标志物。

方法

分析了44例患者的房水：15例原发性开角型青光眼（POAG）患者、14例剥脱性青光眼（PEX）患者和15例对照者（CO）。将房水与牛视网膜抗原的western印迹进行检测。通过多变量统计技术分析IgG抗体模式。通过质谱（基质辅助激光解吸电离飞行时间质谱）鉴定一些重要的生物标志物。

结果

所有患者均表现出针对视网膜抗原的复杂IgG抗体模式。判别分析显示POAG组与CO组的抗体谱有统计学显著差异（p = 0.00018）。PEX组与CO组的抗体谱也有统计学显著差异（p = 0.0013）。与对照组相比，青光眼组不仅观察到上调，也有下调。POAG组和PEX组的抗体模式无显著差异。鉴定出的生物标志物包括热休克蛋白27、α -烯醇化酶、肌动蛋白和甘油醛 - 3 -磷酸脱氢酶。