Blanca M, Mayorga C, Sanchez F, Vega J M, Fernandez J, Juarez C, Suau R, Perez E
Research Unit for Allergic Diseases, Hospital Materno Infantil, Malaga, Spain.
Allergy. 1991 Nov;46(8):632-8. doi: 10.1111/j.1398-9995.1991.tb00635.x.
To determine the influence of the acyl side chain on the IgE antibody specificity of the two most common penicillins inducing allergic reactions in our community, benzylpenicillin (BP) and amoxicillin (AX), IgE positive sera from a group of 24 patients allergic to penicillin were studied. RAST was determined in parallel to benzyl penicilloyl-polylysine (BPO-PLL) and amoxicilloyl-polylysine (AX-PLL) in each serum, and values greater than an established coefficient of variation of 15% were considered as different for either of the haptens used (BP and AX). 16 sera proved to be more positive to BP (Group A), six to AX (Group B) and in two IgE was positive to both haptens with a similar value (Group C). RAST titration concentration effect curves and RAST inhibition studies with a pool of sera from each group (A, B, C) and individual sera showed that most of them were more specific for either BPO or AX, a minority being of similar value to both. These findings indicate that the side chain structure of penicillins is relevant in the constitution of the antigenic determinant, although in some instances the common chemical structure of betalactam is recognised mainly by the IgE antibodies. The clinical relevance of these data needs to be established.
为了确定酰基侧链对在我们社区引起过敏反应的两种最常见青霉素（苄青霉素（BP）和阿莫西林（AX））的IgE抗体特异性的影响，我们研究了一组24名对青霉素过敏患者的IgE阳性血清。在每份血清中同时测定与苄青霉素酰聚赖氨酸（BPO-PLL）和阿莫西林酰聚赖氨酸（AX-PLL）的放射性变应原吸附试验（RAST），对于所使用的任何一种半抗原（BP和AX），大于既定变异系数15%的值被视为不同。16份血清对BP更呈阳性（A组），6份对AX更呈阳性（B组），2份血清中IgE对两种半抗原均呈阳性且值相似（C组）。来自每组（A、B、C）的混合血清和个体血清的RAST滴定浓度效应曲线以及RAST抑制研究表明，大多数血清对BPO或AX更具特异性，少数血清对两者的值相似。这些发现表明，青霉素的侧链结构在抗原决定簇的构成中具有相关性，尽管在某些情况下，β-内酰胺的共同化学结构主要被IgE抗体识别。这些数据的临床相关性有待确定。
