微粒体醛氧化酶活性的荧光测定法：以9-蒽醛为底物。

Fluorometric assay of microsomal aldehyde oxygenase activity: 9-anthraldehyde as a substrate.

作者信息

Watanabe K, Ozaki M, Matsunaga T, Yamamoto I, Yoshimura H

机构信息

Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.

出版信息

Anal Biochem. 1991 Oct;198(1):134-7. doi: 10.1016/0003-2697(91)90517-w.

DOI:10.1016/0003-2697(91)90517-w

PMID:1789414

Abstract

With regard to hepatic microsomal oxidation of 9-anthraldehyde (9-AA), a fluorometric method for determination of 9-anthracene carboxylic acid (9-ACA) is described. 9-AA was incubated with hepatic microsomes prepared from male ddN mice. 9-ACA formed was fluorometrically (excitation and emission wavelengths of 255 and 458 nm, respectively) quantitated after the separation from 9-AA by an alkali extraction and ethyl acetate reextraction. Hepatic microsomes less than 0.1 mg protein were enough to assay the microsomal aldehyde oxidation. The enzyme in the microsomes that catalyzes the oxidation of 9-AA to 9-ACA has been characterized by this method.

摘要

关于9-蒽甲醛（9-AA）的肝微粒体氧化，本文描述了一种测定9-蒽甲酸（9-ACA）的荧光法。将9-AA与从雄性ddN小鼠制备的肝微粒体一起孵育。通过碱萃取和乙酸乙酯再萃取从9-AA中分离出来后，对形成的9-ACA进行荧光定量（激发波长和发射波长分别为255和458nm）。蛋白质含量低于0.1mg的肝微粒体就足以检测微粒体醛氧化。通过该方法对微粒体中催化9-AA氧化为9-ACA的酶进行了表征。