具有扩展线性度和减少干扰的安培型酒精电极。

Amperometric alcohol electrode with extended linearity and reduced interferences.

作者信息

Lubrano G J, Faridnia M H, Palleschi G, Gyilbault G G

机构信息

Universal Sensors, Incorporated, Metairie, Louisiana 70006.

出版信息

Anal Biochem. 1991 Oct;198(1):97-103. doi: 10.1016/0003-2697(91)90512-r.

DOI:10.1016/0003-2697(91)90512-r

PMID:1789436

Abstract

Conventional amperometric alcohol electrodes were constructed with oxygen- and hydrogen peroxide-base sensors and a much improved electrode was designed by placing a hydrophobic, gas-permeable membrane over the conventional hydrogen peroxide-based alcohol electrode. The immobilization of alcohol oxidase with glutaraldehyde was also studied and optimized. The upper linear ranges of the conventional and newly designed alcohol electrodes were 0.02 and 0.5% ethanol, respectively. The hydrophobic membrane of the new design eliminated the classical electrochemical interferences of hydrogen peroxide-based electrodes and the typical pH dependence of enzymatic systems.

摘要

传统的安培型酒精电极是由基于氧气和过氧化氢的传感器构建而成的，通过在传统的基于过氧化氢的酒精电极上放置一层疏水、透气的膜，设计出了一种改进得多的电极。还研究并优化了用戊二醛固定酒精氧化酶的方法。传统酒精电极和新设计的酒精电极的线性上限分别为0.02%和0.5%的乙醇。新设计的疏水膜消除了基于过氧化氢电极的经典电化学干扰以及酶系统典型的pH依赖性。