采用胶束电动毛细管色谱法结合柱上快速扫描多色吸收检测法分析人尿中的非法药物。

Analysis of illicit drugs in human urine by micellar electrokinetic capillary chromatography with on-column fast scanning polychrome absorption detection.

作者信息

Wernly P, Thormann W

机构信息

Department of Clinical Pharmacology, University of Bern, Switzerland.

出版信息

Anal Chem. 1991 Dec 15;63(24):2878-82. doi: 10.1021/ac00024a014.

DOI:10.1021/ac00024a014

PMID:1789451

Abstract

Using micellar electrokinetic capillary chromatography (MECC) with a borate/phosphate buffer containing 75 mM SDS (pH 9.1), common drugs of abuse and/or their metabolites, including opioids, benzoylecgonine, amphetamines, and methaqualone, can easily be analyzed. After solid-phase extraction of 5 mL of urine, drug concentrations down to about 100 ng/mL can be unambiguously monitored with on-column multiwavelength detection. Peak assignment is achieved through comparison of retention times and absorption spectra of eluting peaks with those of computer-stored model runs. The effectiveness of the approach is demonstrated with data obtained from different patient urines which tested positively for one or several drugs using nonisotopic immunoassays. Results suggest that MECC of illicit drugs is a highly specific and sensitive instrumental approach suitable for confirmation testing following a positive response of a toxicological screening procedure.

摘要

使用含有75 mM SDS（pH 9.1）的硼酸盐/磷酸盐缓冲液的胶束电动毛细管色谱法（MECC），可以轻松分析常见的滥用药物和/或其代谢物，包括阿片类药物、苯甲酰芽子碱、苯丙胺和甲喹酮。在对5 mL尿液进行固相萃取后，通过柱上多波长检测可以明确监测低至约100 ng/mL的药物浓度。通过将洗脱峰的保留时间和吸收光谱与计算机存储的模型运行结果进行比较来实现峰鉴定。该方法的有效性通过从不同患者尿液中获得的数据得到证明，这些尿液使用非同位素免疫分析法检测出一种或几种药物呈阳性。结果表明，非法药物的MECC是一种高度特异性和灵敏的仪器方法，适用于毒理学筛查程序呈阳性反应后的确认测试。