Aubuchon James P, Dumont Larry J, Herschel Louise, Roger Jill, Beddard Rachel L, Taylor Harry L, Whitley Pamela H, Sawyer Sherrie L, Graminske Sharon, Martinson Karen, Dora Ross, Heldke Sybil, Adamson John, Rose Leslie E
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA.
Transfusion. 2008 Jan;48(1):147-52. doi: 10.1111/j.1537-2995.2007.01492.x. Epub 2007 Sep 24.
Automated collection of blood components offers multiple advantages and has prompted development of portable devices. This study sought to document the biochemical and hematologic properties and in vivo recovery of red cells (RBCs) collected via a new device that employed a variable-volume centrifugal separation chamber.
Normal subjects (n = 153) donated 2 units of RBCs via an automated blood collection system (Cymbal, Haemonetics). Procedures were conducted with wall outlet power (n = 49) or the device's battery source (n = 104). Units were collected with or without leukoreduction filtration and were stored in AS-3 for 42 days. The units were assessed via standard biochemical and hematologic tests before and after storage, and 24 leukoreduced (LR) and 24 non-LR RBCs were radiolabeled on Day 42 with Na(2)(51)CrO(4) for autologous return to determine recovery at 24 hours with concomitant determination of RBC volume via infusion of (99m)Tc-labeled fresh RBCs.
Two standard RBC units (targeted to contain 180 mL of RBCs plus 100 mL of AS-3) could be collected in 35.7 +/- 2.0 minutes (n = 30) or 40.3 +/- 2.7 minutes for LR RBCs (n = 92). An additional 31 collections were conducted successfully with intentional filter bypassing. RBC units contained 104 +/- 4.1 percent of their targeted volumes (170-204 mL of RBCs), and LR RBCs contained 92 percent of non-LR RBCs' hemoglobin. All LR RBCs contained less than 1 x 10(6) white blood cells. Mean hemolysis was below 0.8 percent (Day 42) for all configurations. Adenosine triphosphate was well preserved. Mean recovery was 82 +/- 4.9 percent for RBCs and 84 +/- 7.0 percent for LR RBCs.
The Cymbal device provided quick and efficient collection of 2 RBC units with properties meeting regulatory requirements and consistent with good clinical utility.
血液成分的自动化采集具有多种优势，并促使了便携式设备的研发。本研究旨在记录通过一种采用可变体积离心分离腔的新设备采集的红细胞（RBC）的生化和血液学特性以及体内回收率。
正常受试者（n = 153）通过自动采血系统（Cymbal，Haemonetics）捐献2单位红细胞。操作使用壁式电源（n = 49）或设备的电池供电（n = 104）。采集的单位血液进行或不进行白细胞滤除，储存于AS-3中42天。在储存前后通过标准生化和血液学检测对单位血液进行评估，24个白细胞滤除（LR）和24个未进行白细胞滤除的红细胞在第42天用Na(2)(51)CrO(4)进行放射性标记以便自体回输，以确定24小时的回收率，并通过输注(99m)Tc标记的新鲜红细胞同时测定红细胞体积。
两个标准红细胞单位（目标为含有180 mL红细胞加100 mL AS-3）可在35.7±2.0分钟（n = 30）内采集完成，白细胞滤除的红细胞则需40.3±2.7分钟（n = 92）。另外31次采集通过故意绕过过滤器成功完成。红细胞单位所含体积为目标体积的104±4.1%（170 - 204 mL红细胞），白细胞滤除的红细胞所含血红蛋白为未进行白细胞滤除的红细胞的92%。所有白细胞滤除的红细胞所含白细胞少于1×10(6)个。所有配置下平均溶血率在第42天低于0.8%。三磷酸腺苷保存良好。红细胞的平均回收率为82±4.9%，白细胞滤除的红细胞为84±7.0%。
Cymbal设备能够快速、高效地采集2单位红细胞，其特性符合监管要求且具有良好的临床实用性。
