AuBuchon James P, Cancelas Jose A, Herschel Louise, Roger Jill, Rugg Neeta, Pratt Patricia G, Joines Amy, Gormas Jennifer F, Zia Majid, Spearman Michael R
Cell Labeling Laboratory, Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA.
Transfusion. 2006 Aug;46(8):1311-5. doi: 10.1111/j.1537-2995.2006.00897.x.
Documentation of the benefits of leukoreduction has led to the increased use of this technique and the need for development of efficient and effective techniques for its accomplishment. This study investigated the in vitro properties and in vivo autologous radiolabeled recovery of leukoreduced red cells (RBCs) produced through a leukoreduction filtration system for RBCs (LEUKOSEP HRC-600-C, Hemerus Medical).
Normal subjects donated 36 units of RBCs that were leukoreduced on Days 0, 3, or 5 through a "hands-off" technique. Biochemical studies were performed before and after filtration and at the end of 42 days of storage. Units leukoreduced on Days 0 or 5 were held until Day 42 and used for autologous radiolabeled return to determine recovery with 51Cr single-label radiolabeling techniques.
Leukoreduction filtration was accomplished in 16.3 +/- 2 minutes on Day 0 at room temperature or 27 to 30 minutes on Days 3 or 5 after refrigeration. Leukoreduction efficiency was 4.6 +/- 0.6 log with a median residual white blood cell (WBC) content of fewer than 3.3 x 10(4) WBCs per unit. RBC recovery was 90 +/- 2 percent. Hemolysis was 0.34 +/- 0.16 percent at the end of 42 days of storage. The in vivo recovery of radiolabeled RBCs 24 hours after autologous return was 80.6 +/- 4.5 percent for RBC units leukoreduced on Days 0 and 5 combined.
The LEUKOSEP HRC-600-C WBC reduction filtration system produced leukoreduced RBCs efficiently and effectively with acceptable poststorage biochemical measures and posttransfusion recovery after 42 days of storage.
白细胞去除术益处的记录促使该技术的使用增加，并且需要开发高效且有效的实现技术。本研究调查了通过红细胞白细胞去除过滤系统（LEUKOSEP HRC - 600 - C，Hemerus Medical公司）生产的白细胞去除红细胞（RBC）的体外特性和体内自体放射性标记恢复情况。
正常受试者捐献了36单位红细胞，通过“免接触”技术在第0天、第3天或第5天进行白细胞去除。在过滤前后以及储存42天结束时进行生化研究。在第0天或第5天进行白细胞去除的单位保存至第42天，并用于自体放射性标记回输，以通过51Cr单标记放射性标记技术确定恢复情况。
在室温下，第0天白细胞去除过滤在16.3±2分钟内完成；在第3天或第5天冷藏后，需要27至30分钟。白细胞去除效率为4.6±0.6对数，每单位残留白细胞（WBC）含量中位数少于3.3×10⁴个WBC。红细胞回收率为90±2%。储存42天结束时溶血率为0.34±0.16%。第0天和第5天进行白细胞去除的红细胞单位自体回输24小时后，放射性标记红细胞的体内回收率为80.6±4.5%。
LEUKOSEP HRC - 600 - C白细胞去除过滤系统能高效且有效地生产白细胞去除红细胞，储存后生化指标可接受，储存42天后输血后恢复情况良好。
