Coert B A, Anderson R E, Meyer F B
J Stroke Cerebrovasc Dis. 1999 Nov-Dec;8(6):380-7. doi: 10.1016/s1052-3057(99)80045-7.
Although middle cerebral artery (MCA) occlusion in the rat is often used to study focal cerebral ischemia, the model of ischemia affects the size and reproducibility of infarction. The purpose of this experiment was to methodically examine different preparations to determine the optimum focal cerebral ischemia model to produce a reproducible severe ischemic injury. Eighty-two Wistar rats underwent either 1 hour, 3 hour, or permanent MCA occlusion combined with no, unilateral, or bilateral common carotid artery artery (CCA) occlusion. Three days after ischemia, the animals were prepared for tetrazolium chloride assessment of infarction size. One-hour MCA occlusion produced a coefficient of variation (CV) of 200% with an infarction volume of 20.3+/-10.5 mm(3). Adding unilateral or bilateral CCA occlusion resulted in a CV of 134% and 101%, respectively. Three-hour MCA occlusion combined with bilateral CCA occlusion decreased the CV to 58% with a cortical infarction volume of 82.6+/-12.1 mm(3), P<05, compared with 1-hour MCA occlusion with or without CCA occlusion. Permanent MCA occlusion combined with 3 hours of bilateral CCA occlusion resulted in a CV of 47% with a cortical infarction volume of 89.6+/-16.0 mm(3). These results indicate that 3-hour MCA occlusion combined with bilateral CCA occlusion provide consistently a large infarction volume after temporary focal cerebral ischemia.
尽管大鼠大脑中动脉（MCA）闭塞常用于研究局灶性脑缺血，但缺血模型会影响梗死灶的大小和可重复性。本实验的目的是系统地检查不同的制备方法，以确定能产生可重复的严重缺血性损伤的最佳局灶性脑缺血模型。82只Wistar大鼠接受了1小时、3小时或永久性MCA闭塞，同时分别不进行、单侧或双侧颈总动脉（CCA）闭塞。缺血三天后，对动物进行氯化三苯基四氮唑评估梗死灶大小。1小时MCA闭塞产生的变异系数（CV）为200%，梗死体积为20.3±10.5立方毫米。添加单侧或双侧CCA闭塞后，CV分别为134%和101%。3小时MCA闭塞联合双侧CCA闭塞使CV降至58%，皮质梗死体积为82.6±12.1立方毫米，与1小时MCA闭塞无论有无CCA闭塞相比，P<0.05。永久性MCA闭塞联合3小时双侧CCA闭塞导致CV为47%，皮质梗死体积为89.6±16.0立方毫米。这些结果表明，3小时MCA闭塞联合双侧CCA闭塞在短暂性局灶性脑缺血后始终能产生较大的梗死体积。
