Vicini D S, Ogilvie G K, Katzenellenbogen J, Carlson K
Department of Veterinary Clinical Sciences, University of Illinois, Urbana 61801.
Am J Vet Res. 1991 Dec;52(12):2055-9.
Estrogen receptors were measured in normal canine lymph nodes and neoplastic tissue from dogs with lymphoma, using a commercially available [3H]estradiol dextran-coated charcoal assay. Using the same assay, estrogen receptors were detected in the positive-control tissues--dog uterus, rat uterus, and lyophilized bovine uterus. Specific binding of [3H]estradiol was not detected in rat skeletal muscle or in any of the canine lymphoid tissues, indicating that the specimens did not contain estrogen receptors.
使用市售的[3H]雌二醇葡聚糖包被活性炭分析法，对正常犬淋巴结以及患有淋巴瘤的犬的肿瘤组织中的雌激素受体进行了检测。采用相同的分析方法，在阳性对照组织——犬子宫、大鼠子宫和冻干牛子宫中检测到了雌激素受体。在大鼠骨骼肌或任何犬类淋巴组织中均未检测到[3H]雌二醇的特异性结合，这表明这些标本中不含有雌激素受体。
