Kent Clement, Azanchi Reza, Smith Ben, Chu Adrienne, Levine Joel
Department of Biology, University of Toronto at Mississauga, Mississauga, Ontario, Canada.
PLoS One. 2007 Sep 26;2(9):e962. doi: 10.1371/journal.pone.0000962.
Drosophila Cuticular Hydrocarbons (CH) influence courtship behaviour, mating, aggregation, oviposition, and resistance to desiccation. We measured levels of 24 different CH compounds of individual male D. melanogaster hourly under a variety of environmental (LD/DD) conditions. Using a model-based analysis of CH variation, we developed an improved normalization method for CH data, and show that CH compounds have reproducible cyclic within-day temporal patterns of expression which differ between LD and DD conditions. Multivariate clustering of expression patterns identified 5 clusters of co-expressed compounds with common chemical characteristics. Turnover rate estimates suggest CH production may be a significant metabolic cost. Male cuticular hydrocarbon expression is a dynamic trait influenced by light and time of day; since abundant hydrocarbons affect male sexual behavior, males may present different pheromonal profiles at different times and under different conditions.
果蝇表皮碳氢化合物（CH）会影响求偶行为、交配、聚集、产卵以及抗干燥能力。我们在多种环境（光照/黑暗）条件下，每小时测量了单个雄性黑腹果蝇体内24种不同CH化合物的水平。通过基于模型的CH变异分析，我们开发了一种改进的CH数据归一化方法，并表明CH化合物在一天内具有可重复的循环表达时间模式，且在光照和黑暗条件下有所不同。表达模式的多变量聚类识别出了5组具有共同化学特征的共表达化合物。周转率估计表明CH的产生可能是一项重大的代谢成本。雄性表皮碳氢化合物的表达是一种受光照和一天中的时间影响的动态特征；由于丰富的碳氢化合物会影响雄性性行为，雄性可能在不同时间和不同条件下呈现不同的信息素谱。
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