CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences (CAS), Shanghai, China.
CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China.
Science. 2021 Jan 22;371(6527):411-415. doi: 10.1126/science.abd4359.
mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes () and () in the head of field-caught swarming males. Knockdown of and expression affects and male mating in the laboratory, and it reduces male swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating and/or expression. Moreover, the desaturase gene is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.
交配是由雄性在黄昏时分的群集行为引发的，随后雌性飞入群集。在这里，我们表明蚊子的群集和交配是由生物钟基因、光照和温度协调指导的。转录组分析显示，野外捕获的群集雄性头部的生物钟基因 () 和 () 上调。和 表达的敲低会影响实验室中 和 雄性的交配，并且在半野外条件下会减少雄性的群集和交配。光照和温度会影响蚊子的交配，可能是通过调节 和/或 的表达。此外，脱饱和酶基因 在群集雄性的头部上调和节律性表达，并调节表皮碳氢化合物的产生，包括二十七烷，它刺激交配活动。
