[泛素在大鼠皮肤切割伤愈合过程中的表达及其法医学意义]
[The expression of ubiquitin in the healing process of incised wounds of rat's skin and its significance to forensic medicine].
作者信息
Zeng Yi, Deng Shi-xiong
机构信息
Department of Forensic Medicine, Chongqing university of Medical Sciences, Chongqing 400016, China.
出版信息
Fa Yi Xue Za Zhi. 2007 Aug;23(4):241-3.
OBJECTIVE
To evaluate the changes of ubiquitin expression in incised wounds of the rat skin.
METHODS
The testing rat groups were subjected to incised skin wounds, with normal rat skin used as control. The expression level of ubiquitin was assessed using immunohistochemistry and imaging analysis technique on skin samples taken at 1, 3, 6, 12h, and on day 1, 3, 6, 10, and 14d after injury.
RESULTS
The expression level of ubiquitin was low in the skin of normal control group. Increased level of ubiquitin expression could be observed 1 h after injury. The expression level of ubiquitin reached its peak on day 6 and started to decline on day 10, and then returned to its normal level on day 14 d after injury.
CONCLUSION
Ubiquitin may serve as a potentially useful marker for forensic determination of the skin wound age.
目的
评估大鼠皮肤切割伤中泛素表达的变化。
方法
将实验大鼠分组制作皮肤切割伤，以正常大鼠皮肤作为对照。采用免疫组织化学和图像分析技术，对伤后1、3、6、12小时以及伤后第1、3、6、10和14天采集的皮肤样本进行泛素表达水平评估。
结果
正常对照组皮肤中泛素表达水平较低。伤后1小时即可观察到泛素表达水平升高。泛素表达水平在第6天达到峰值，第10天开始下降，伤后第14天恢复至正常水平。
结论
泛素可能作为法医学判定皮肤伤口损伤时间的一个潜在有用标志物。
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