Li Xiaodong, Li Xiaohui, Zhang Jianxiang, Zhao Shifang, Shen Jiacong
Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310027, China.
J Biomed Mater Res A. 2008 Jun 1;85(3):768-76. doi: 10.1002/jbm.a.31543.
Novel "micelles enhanced" polyelectrolyte (PE) capsules based on functional templates of hybrid calcium carbonate were fabricated. Evidences suggested that the structure of capsule wall was different from that of conventional PE capsules, and the wall permeability of these PE capsules changed significantly. Lysozyme, a positively charged protein in neutral solution, was studied as a model protein to be encapsulated into the "micelles enhanced" PE capsules. Confocal laser scanning microscope was used to observe the entrapping process in real time, while UV-Vis spectroscope and scanning force microscope measurements suggested the high efficiency of encapsulation. In addition, the fluorescence recovery after photobleaching technique was employed to determine the existence form of deposited molecules. Further studies showed even negatively charged water-soluble peptides or proteins can be encapsulated into these hybrid capsules by modulating the pH value in bulk solution under its isoelectronic point as well.
基于杂化碳酸钙功能模板制备了新型“胶束增强”聚电解质（PE）胶囊。有证据表明，胶囊壁的结构与传统PE胶囊不同，且这些PE胶囊的壁通透性发生了显著变化。溶菌酶是一种在中性溶液中带正电荷的蛋白质，作为模型蛋白被研究用于包封到“胶束增强”PE胶囊中。利用共聚焦激光扫描显微镜实时观察包封过程，而紫外可见光谱仪和扫描力显微镜测量结果表明包封效率很高。此外，采用光漂白后荧光恢复技术来确定沉积分子的存在形式。进一步研究表明，通过在等电点以下调节本体溶液的pH值，即使是带负电荷的水溶性肽或蛋白质也能被包封到这些杂化胶囊中。
