DeQueiroz G A, Day D F
Audubon Sugar Institute, Louisiana State University Agricultural Center, St. Gabriel, LA 70776, USA.
J Appl Microbiol. 2007 Oct;103(4):794-802. doi: 10.1111/j.1365-2672.2007.03299.x.
To evaluate both the antimicrobial activity and the effectiveness of a combination of sodium hypochlorite and hydrogen peroxide (Ox-B) for killing Pseudomonas aeruginosa ATCC 19142 cells and removing P. aeruginosa biofilms on aluminum or stainless steel surfaces.
Pseudomonas aeruginosa biofilms were developed in tryptic soy broth containing vertically suspended aluminium or stainless steel plates. Biofilms were exposed to a mixed sodium hypochlorite and hydrogen peroxide solution as a sanitizer for 1, 5 and 20 min. The sanitizer was then neutralized, the cells dislodged from the test surfaces, and viable cells enumerated. Cell morphologies were determined using scanning (SEM) and transmission electron microscopy (TEM). Cell viability was determined by confocal scanning laser microscopy (CSLM). Biofilm removal was monitored by Fourier transform infrared (FTIR) spectrophotometry. Cell numbers were reduced by 5-log to 6-log after 1 min exposure and by 7-log after 5 min exposure to Ox-B. No viable cells were detected after a 20 min exposure. Treatment with equivalent concentrations of sodium hypochlorite reduced viable numbers by 3-log to 4-log after 1 min exposure and by 4-log to 6-log after 5 min, respectively. A 20 min exposure achieved a 7-log reduction. Hydrogen peroxide at test concentration treatments showed no effect. FTIR analysis of treated pseudomonad biofilms on aluminium or stainless steel plates showed either a significant reduction or complete removal of biofilm material after a 5 min exposure to the mixed sodium hypochlorite and hydrogen peroxide solution. SEM and TEM images revealed damage to cell wall and cell membranes.
A combination of sodium hypochlorite and hydrogen peroxide effectively killed P. aeruginosa cells and removed biofilms from both stainless steel and aluminium surfaces.
The combination of sodium hypochlorite and hydrogen peroxide can be used as an alternative disinfectant and/or biofilm remover of contaminated food processing equipment.
评估次氯酸钠和过氧化氢组合剂（Ox - B）对杀灭铜绿假单胞菌ATCC 19142细胞以及去除铝或不锈钢表面铜绿假单胞菌生物膜的抗菌活性和有效性。
在含有垂直悬挂的铝或不锈钢板的胰蛋白胨大豆肉汤中形成铜绿假单胞菌生物膜。将生物膜暴露于次氯酸钠和过氧化氢混合溶液作为消毒剂中1、5和20分钟。然后中和消毒剂，从测试表面去除细胞，并对活细胞进行计数。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）确定细胞形态。通过共聚焦扫描激光显微镜（CSLM）确定细胞活力。通过傅里叶变换红外（FTIR）分光光度法监测生物膜去除情况。暴露于Ox - B 1分钟后细胞数量减少5个对数至6个对数，暴露5分钟后减少7个对数。暴露20分钟后未检测到活细胞。用等效浓度的次氯酸钠处理，暴露1分钟后活细胞数量分别减少3个对数至4个对数，暴露5分钟后减少4个对数至6个对数。暴露20分钟实现7个对数的减少。测试浓度的过氧化氢处理无效果。对铝或不锈钢板上经处理的假单胞菌生物膜进行FTIR分析表明，暴露于次氯酸钠和过氧化氢混合溶液5分钟后，生物膜物质显著减少或完全去除。SEM和TEM图像显示细胞壁和细胞膜受损。
次氯酸钠和过氧化氢组合剂能有效杀灭铜绿假单胞菌细胞，并从不锈钢和铝表面去除生物膜。
次氯酸钠和过氧化氢组合剂可作为受污染食品加工设备的替代消毒剂和/或生物膜去除剂。
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