DeQueiroz G A, Day D F
Audubon Sugar Institute, Louisiana State University Agricultural Center, St. Gabriel, Louisiana 70776, USA.
Lett Appl Microbiol. 2008 Feb;46(2):176-80. doi: 10.1111/j.1472-765X.2007.02283.x.
To evaluate a sodium hypochlorite and hydrogen peroxide solution (Ox-B7) as a potential decontaminant of Bacillus subtilis spore-contaminated surface materials (porous and nonporous).
Test materials were contaminated with B. subtilis spores to a final concentration in the range of 5.7-6.6 log CFU cm(-2). Ox-B7 reduced spore counts by 99.999% (5 log) for both porous and nonporous surfaces within a 5-min contact. Treatment with equivalent concentrations of only sodium hypochlorite reduced spore counts by 99% (2 log) on porous materials and by 99.99% (4 log) on nonporous materials. Hydrogen peroxide treatments reduced spores by less than 90% (<1 log) on both porous and nonporous materials when compared with untreated samples.
A combination of sodium hypochlorite and hydrogen peroxide (Ox-B7) effectively killed B. subtilis spores on both porous and nonporous surface materials.
The combination of sodium hypochlorite and hydrogen peroxide can be used as an alternative disinfectant of spore-contaminated surface materials, as it is more effective than when hydrogen peroxide or sodium hypochlorite are used separately.
评估次氯酸钠和过氧化氢溶液（Ox-B7）作为枯草芽孢杆菌孢子污染的表面材料（多孔和无孔）潜在去污剂的效果。
测试材料被枯草芽孢杆菌孢子污染，最终浓度在5.7 - 6.6 log CFU cm(-2)范围内。Ox-B7在5分钟接触时间内，使多孔和无孔表面的孢子数量减少99.999%（5个对数级）。仅用等效浓度的次氯酸钠处理，使多孔材料上的孢子数量减少99%（2个对数级），无孔材料上减少99.99%（4个对数级）。与未处理的样品相比，过氧化氢处理使多孔和无孔材料上的孢子减少均不到90%（<1个对数级）。
次氯酸钠和过氧化氢的组合（Ox-B7）能有效杀灭多孔和无孔表面材料上的枯草芽孢杆菌孢子。
次氯酸钠和过氧化氢的组合可作为孢子污染表面材料的替代消毒剂，因为它比单独使用过氧化氢或次氯酸钠更有效。
