Barros G G, Chiotta M L, Reynoso M M, Torres A M, Chulze S N
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
J Appl Microbiol. 2007 Oct;103(4):900-9. doi: 10.1111/j.1365-2672.2007.03312.x.
The objectives of this study were: (i) to evaluate genetic relatedness among Aspergillus section Flavi strains isolated from soil and peanut seeds in Argentina; (ii) to determine if AFLP molecular markers could be useful to identify isolates up to species level, and to correlate these markers with the isolates' toxigenic potentials and/or vegetative compatibility group (VCG) affiliations.
Amplified fragment length polymorphism (AFLPs) analysis was applied to compare 82 isolates of Aspergillus section Flavi. Cluster analysis showed a clear separation of A. flavus and A. parasiticus, and comparison of fingerprints revealed several specific markers for each group of isolates. AFLP analysis indicates that no genotypical differences can be established between aflatoxigenic and nonaflatoxigenic producers in both species analysed. In addition, candidate AFLP markers associated with a particular VCG were not found.
There was a concordance between morphological identification and separation up to species level using molecular markers. The findings of specific bands for A. flavus and A. parasiticus may be useful for the design of specific PCR primers in order to differentiate these species and detect them in food.
The present study provides new data on molecular characterization of Aspergillus section Flavi in Argentina.
本研究的目标是：（i）评估从阿根廷土壤和花生种子中分离出的黄曲霉群菌株之间的遗传相关性；（ii）确定扩增片段长度多态性（AFLP）分子标记是否有助于在种水平上鉴定分离株，并将这些标记与分离株的产毒潜力和/或营养体亲和群（VCG）归属相关联。
应用扩增片段长度多态性（AFLP）分析比较了82株黄曲霉群分离株。聚类分析显示黄曲霉和寄生曲霉明显分离，指纹图谱比较揭示了每组分离株的几个特异性标记。AFLP分析表明，在所分析的两个物种中，产黄曲霉毒素和不产黄曲霉毒素的菌株之间无法确定基因型差异。此外，未发现与特定VCG相关的候选AFLP标记。
形态学鉴定与使用分子标记在种水平上的分离结果一致。黄曲霉和寄生曲霉特异性条带的发现可能有助于设计特异性PCR引物，以便区分这些物种并在食品中检测它们。
本研究提供了关于阿根廷黄曲霉群分子特征的新数据。
