Xu Yang, Arai Hidenori, Murayama Toshinori, Kita Toru, Yokode Masayuki
Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Biochem Biophys Res Commun. 2007 Nov 23;363(3):782-7. doi: 10.1016/j.bbrc.2007.09.029. Epub 2007 Sep 19.
Recently, the role of bone marrow (BM)-derived endothelial cells and smooth muscle cells (SMCs) has been extensively studied in the pathogenesis of atherosclerosis. In this study we examined the effect of hypercholesterolemia on cuff-induced intimal thickening in LDL-receptor knockout (LDLR-/-) mice fed with a high-fat diet. We transplanted BM of green fluorescence protein (GFP)-transgenic mice to LDLR-/- mice to identify the cell lineage in the lesion. After BM transplantation mice were fed with a high-fat diet for 4 weeks and were then planted a polyethylene cuff on the right femoral artery. Two weeks after cuff placement, atherosclerotic lesions developed in the intima predominantly consisting of a massive accumulation of foam cells with a number of alpha smooth muscle actin (alphaSMA)- and GFP-positive cells. Adventitial small vessels were positive both for CD31 and GFP. Our data indicate that BM-derived cells can contribute to the development of atherosclerosis in the presence of hypercholesterolemia.
最近，骨髓（BM）来源的内皮细胞和平滑肌细胞（SMC）在动脉粥样硬化发病机制中的作用已得到广泛研究。在本研究中，我们检测了高胆固醇血症对喂食高脂饮食的低密度脂蛋白受体敲除（LDLR-/-）小鼠袖带诱导内膜增厚的影响。我们将绿色荧光蛋白（GFP）转基因小鼠的骨髓移植到LDLR-/-小鼠体内，以确定病变中的细胞谱系。骨髓移植后，小鼠喂食高脂饮食4周，然后在右股动脉植入一个聚乙烯袖带。袖带放置两周后，内膜出现动脉粥样硬化病变，主要由大量泡沫细胞聚集以及一些α平滑肌肌动蛋白（αSMA）和GFP阳性细胞组成。外膜小血管CD31和GFP均呈阳性。我们的数据表明，在高胆固醇血症存在的情况下，骨髓来源的细胞可促进动脉粥样硬化的发展。
