Williams Geoffrey R, Shafer Aaron B A, Rogers Richard E L, Shutler Dave, Stewart Donald T
Department of Biology, Acadia University, Wolfville, NS, Canada B4P 2R6.
J Invertebr Pathol. 2008 Feb;97(2):189-92. doi: 10.1016/j.jip.2007.08.005. Epub 2007 Aug 24.
Nosema ceranae is an emerging microsporidian parasite of European honey bees, Apis mellifera, but its distribution is not well known. Six Nosema-positive samples (determined from light microscopy of spores) of adult worker bees from Canada (two each from Nova Scotia, New Brunswick, and Prince Edward Island) and two from USA (Minnesota) were tested to determine Nosema species using previously-developed PCR primers of the 16S rRNA gene. We detected for the first time N. ceranae in Canada and central USA. One haplotype of N. ceranae was identified; its virulence may differ from that of other haplotypes.
蜜蜂微孢子虫是欧洲蜜蜂(西方蜜蜂)新出现的一种微孢子虫寄生虫,但其分布情况尚不清楚。对来自加拿大(新斯科舍省、新不伦瑞克省和爱德华王子岛各两份)的成年工蜂的6份微孢子虫阳性样本(通过孢子光学显微镜检查确定)以及来自美国(明尼苏达州)的2份样本进行了检测,使用先前开发的16S rRNA基因PCR引物来确定微孢子虫的种类。我们首次在加拿大和美国中部检测到了蜜蜂微孢子虫。鉴定出了蜜蜂微孢子虫的一种单倍型;其毒力可能与其他单倍型不同。