Gravet A, Camdessoucens G, Murbach V, Barrand P, Boucher A, Boulenc A, De Briel D, Delarbre J-M, Drzewinski J-C, Flipo J-L, Gherardi C, Grawey I, Gueudet T, Heidt A, Herzig V, Izraelewicz D, Jehl F, Kientz P, Lantz V, Lemble C, Pierrot P, Rieder C, Riehm D, Tytgat F
Laboratoire de microbiologie, hôpital Emile-Muller, 20, avenue du Docteur-Laennec, 68070 Mulhouse cedex, France.
Pathol Biol (Paris). 2007 Nov;55(8-9):424-8. doi: 10.1016/j.patbio.2007.07.019. Epub 2007 Sep 25.
Between 1st January 2005 and 31st December 2005, 232 strains of Streptococcus pneumoniae were collected in the Alsace county from participating laboratories (one from university hospital, 7 from general hospitals and 12 private laboratories) to assess their susceptibility to penicillin and evaluated serogroups of strains.
The coordinating centre performed MICs by the reference agar dilution test, interpreted according to CA-SFM breakpoints. Others antibiotics (erythromycin, cotrimoxazole, tetracycline...) were tested by agar diffusion, ATB-PNEUMO gallery or VITEK gallery (BioMérieux, France) by each participating laboratory. Data were processed, using 4th dimension software.
Strains were collected from 151 blood samples, 38 ear pus, 11 cerebrospinal fluids, 8 pleural liquids and 24 representative pulmonary samples. The prevalence of pneumococci with decreased susceptibility to penicillin G (PDSP) is 35.1% (pulmonary samples excluded). The rate of PNSP decreases for all types of samples compared with other years of surveillance 2003 (44.0%). The rate of blood samples decreases for first time between the creation of Pneumococcal Observatory. The high-level resistance tend to decrease and began low. The PDSP are rather resistant to erythromycin, cotrimoxazole and fosfomycin. Among the PDSP, the most prevalent serotypes were 14, 19, 6 and 9.
Among pneumococcal strains, the rate of PDSP tend however to decrease in 2005 compared with 2003. The rate stays inferior to the observed rates in other French counties where the same decreasing is described.
在2005年1月1日至2005年12月31日期间，从阿尔萨斯县参与的实验室（1所大学医院、7所综合医院和12所私立实验室）收集了232株肺炎链球菌，以评估它们对青霉素的敏感性，并对菌株的血清群进行评估。
协调中心通过参考琼脂稀释试验进行最低抑菌浓度（MIC）测定，并根据CA-SFM标准进行解读。其他抗生素（红霉素、复方新诺明、四环素……）由各参与实验室通过琼脂扩散法、ATB-肺炎链球菌鉴定板或VITEK鉴定板（法国生物梅里埃公司）进行检测。数据使用第四维度软件进行处理。
菌株来自151份血液样本、38份耳脓样本、11份脑脊液样本、8份胸水样本和24份代表性肺样本。对青霉素G敏感性降低的肺炎球菌（PDSP）患病率为35.1%（不包括肺样本）。与2003年其他监测年份相比，所有类型样本中青霉素不敏感肺炎球菌（PNSP）的比例均有所下降（2003年为44.0%）。自肺炎球菌监测站设立以来，血液样本中的比例首次下降。高水平耐药性趋于下降且开始时较低。PDSP对红霉素、复方新诺明和磷霉素具有相当的耐药性。在PDSP中，最常见的血清型为14、19、6和9。
与2003年相比，2005年肺炎球菌菌株中PDSP的比例趋于下降。该比例仍低于法国其他描述了相同下降情况的县所观察到的比例。
