Iliakis G
Thomas Jefferson University, Department of Radiation Oncology and Nuclear Medicine, Philadelphia, PA 19107.
Bioessays. 1991 Dec;13(12):641-8. doi: 10.1002/bies.950131204.
A widely accepted assumption in radiobiology is that ionizing radiation kills cells by inducing forms of damage in DNA structures that lead to the formation of lethal chromosome aberrations. One goal of radiation biology research is the identification of these forms of DNA damage, the characterization of the mechanisms involved in their repair and the elucidation of the processes involved in their transformation to chromosome damage. In recent years, evidence has accumulated implicating DNA double stranded breaks as lesions relevant for cell killing. Here, the available information on this topic is reviewed together with the methods most commonly used to quantitate induction and repair of this type of lesion. The presentation concludes with an outline of present research directions and future goals.
放射生物学中一个被广泛接受的假设是，电离辐射通过在DNA结构中诱导损伤形式来杀死细胞，这些损伤会导致致死性染色体畸变的形成。放射生物学研究的一个目标是识别这些DNA损伤形式，表征其修复所涉及的机制，并阐明其转化为染色体损伤所涉及的过程。近年来，越来越多的证据表明DNA双链断裂是与细胞杀伤相关的损伤。在此，将对该主题的现有信息以及最常用于定量此类损伤的诱导和修复的方法进行综述。本文最后概述了当前的研究方向和未来目标。
