Nakamura K, Ikeda Y, Iwakami N, Hibino Y, Sugano N
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Biochem Int. 1991 Sep;25(2):355-62.
A highly repetitive component in rat nuclear DNA was isolated by HindIII digestion and cloned. A 370-bp cloned component was highly AT-rich (68.3%) in about one third of the region from the 3'-terminus and showed an anomalously slow gel electrophoretic mobility (k-factor = 1.19). These results indicated that a sequence-directed bending of the helix axis occurs in the component. Accordingly, a subclone containing a tandem dimer of the component was isolated and subjected to a circular permutation analysis for exploring the bend center (1). In consequence, the center was shown to be present in the sequence ranging from position near 270 to the 3'-terminus and estimated to be located around position 340.
通过HindIII酶切和克隆，分离出大鼠核DNA中的一个高度重复成分。一个370 bp的克隆成分在从3'末端起约三分之一的区域内富含AT（68.3%），并且在凝胶电泳中显示出异常缓慢的迁移率（k因子 = 1.19）。这些结果表明该成分中发生了螺旋轴的序列定向弯曲。因此，分离出一个包含该成分串联二聚体的亚克隆，并对其进行环形置换分析以探索弯曲中心（1）。结果表明，弯曲中心位于从靠近270位到3'末端的序列中，估计位于340位左右。
