Totani Nagao, Ojiri Yuko
Faculty of Nutrition, Kobe-Gakuin University, Kobe, Japan.
J Oleo Sci. 2007;56(10):543-51. doi: 10.5650/jos.56.543.
Male Wistar rats were fed ad libitum for 12 weeks a powdered diet (AIN93G; no fat) containing 7 wt% of fresh oil (control), and frying oils heated for 20 h at 180 degrees C with amino acids, gluten, sugar, and wheat starch, respectively. The rats were subjected to anthropometric measurements, hematological analyses, and observations of the liver and kidneys. All of the rats grew well, and no gross symptoms attributable to the experimental oils were observed. The serum of all the experimental groups showed a tendency toward lower levels of triacylglycerol and free fatty acids and higher levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) than that of the control group. Among experimental groups, the rats fed a diet containing oil heated with gluten were least influenced by thermally deteriorated oil in terms of serum levels of glucose, triacylglycerol, phospholipids, cholesterol, and insulin; histological evaluations; and number of dark-red patches found on the surface of the liver.
雄性Wistar大鼠随意进食含7 wt%新鲜油（对照）的粉状饲料（AIN93G；无脂肪）12周，以及分别与氨基酸、麸质、糖和小麦淀粉一起在180℃加热20小时的煎炸油。对大鼠进行人体测量、血液学分析以及肝脏和肾脏观察。所有大鼠生长良好，未观察到可归因于实验用油的明显症状。所有实验组的血清中三酰甘油和游离脂肪酸水平有降低趋势，天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平高于对照组。在各实验组中，就血清葡萄糖、三酰甘油、磷脂、胆固醇和胰岛素水平、组织学评估以及肝脏表面发现的暗红色斑块数量而言，喂食含与麸质一起加热的油的饲料的大鼠受热劣化油的影响最小。
