Seo Mi Jin, Shin Jong Hee, Sohn Jae Keun
Department of Agronomy, Kyungpook National University, Daegu, Republic of Korea.
Cryo Letters. 2007 May-Jun;28(3):207-13.
A simplified technique for the cryoprotection of dormant shoot-tips was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest post-thaw regrowth level was obtained from shoot-tips desiccated by air drying for 5 h. The post-thaw regrowth of P. lactiflora. var. 'Mikang' was the highest (74.9 percent) among the five genotypes tested. The regeneration level after cryopreservation was highest (66approximate74%; average of 70 percent) for dormant shoot-tips collected from November to February. Shoot tips which were dissected from non-dormant buds in late March, however, showed very low post-thaw regrowth. Post-thaw regrowth of shoot-tips was promoted in MS medium containing 1 mg 1(-1) of gibberellic acid (GA3) and 0.5 mg 1(-1) of N(6)-benzyladenine (BA). The elongated shoots were rooted on a 1/4 MS medium containing 0.1 mg 1(-1) of beta-naphthalene acetic acid (NAA). In addition, random amplified polymorphic DNAs (RAPDs) assays suggested that the cryostorage treatment used here preserved the genetic fidelity of the peony dormant shoot-tips. This cryopreservation method appears to be promising for the conservation of herbaceous peony germplasm.
为了对芍药（Paeonia lactiflora Pall.）种质进行保存，开发了一种简化的休眠茎尖冷冻保护技术。通过空气干燥5小时脱水的茎尖解冻后获得了最高的再生长水平。在测试的五个基因型中，‘米康’芍药的解冻后再生长率最高（74.9%）。11月至2月采集的休眠茎尖冷冻保存后的再生水平最高（66%至74%；平均70%）。然而，3月下旬从非休眠芽中切取的茎尖解冻后的再生长率非常低。在含有1 mg/L赤霉素（GA3）和0.5 mg/L N⁶-苄基腺嘌呤（BA）的MS培养基中，茎尖的解冻后再生长得到促进。伸长的茎在含有0.1 mg/Lβ-萘乙酸（NAA）的1/4 MS培养基上生根。此外，随机扩增多态性DNA（RAPD）分析表明，这里使用的冷冻保存处理保持了芍药休眠茎尖的遗传稳定性。这种冷冻保存方法对于芍药种质的保存似乎很有前景。
