Porta Helena, Figueroa-Balderas Rosa Elia, Rocha-Sosa Mario
Departmento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos, C. P. 6100, Mexico.
Planta. 2008 Jan;227(2):363-73. doi: 10.1007/s00425-007-0623-y. Epub 2007 Sep 26.
Chloroplastic LOXs are implicated in the biosynthesis of oxylipins like jasmonic acid and C6 volatiles among others. In this study, we isolated the cDNA of a novel chloroplast-targeted Phaseolus vulgaris LOX, (PvLOX6). This gene is highly induced after wounding, non-host pathogen infection, and by signaling molecules as H2O2, SA, ethylene and MeJA. The phylogenetic analysis of PvLOX6 showed that it is closely related to chloroplast-targeted LOX from potato (H1) and tomato (TomLOXC); both of them are implicated in the biosynthesis of C6 volatiles. Induction of PvLOX6 mRNA by wounding ethylene and jasmonic acid on the one side, and non-host pathogen, salicylic acid on the other indicates that common bean uses the same LOX to synthesize oxylipins in response to different stresses.
叶绿体脂氧合酶参与茉莉酸和C6挥发性物质等氧脂的生物合成。在本研究中，我们分离了一种新的定位于叶绿体的菜豆脂氧合酶（PvLOX6）的cDNA。该基因在创伤、非寄主病原体感染以及受到H2O2、水杨酸、乙烯和茉莉酸甲酯等信号分子刺激后高度诱导表达。PvLOX6的系统发育分析表明，它与马铃薯（H1）和番茄（TomLOXC）中定位于叶绿体的脂氧合酶密切相关；这两种脂氧合酶都参与C6挥发性物质的生物合成。一方面，创伤、乙烯和茉莉酸诱导PvLOX6 mRNA表达，另一方面，非寄主病原体、水杨酸诱导其表达，这表明菜豆在应对不同胁迫时使用相同的脂氧合酶来合成氧脂。
