Martí Joaquín, Santa-Cruz M C, Bayer Shirley A, Ghetti Bernardino, Hervás José P
Departament de Biologia Cellular de Fisiologia i d'Immunologia, Unidad de Citología e Histología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain.
Brain Struct Funct. 2007 Dec;212(3-4):347-57. doi: 10.1007/s00429-007-0159-8. Epub 2007 Sep 25.
Generation and settling of Purkinje cells (PCs) are investigated in the weaver mouse cerebellum in order to determine possible relationships with the fissuration pattern. Tritiated thymidine was supplied to pregnant females at the time that these neurons were being produced. Autoradiography was then applied on brain sections obtained from control and weaver offspring at postnatal (P) day 90. This makes it possible to assess the differential survival of neurons born at distinct embryonic times on the basis of the proportion of labeled cells located at the two foliar compartments: fissures and foliar crowns. Our data show that throughout the surface contour of the vermal lobes, generative programs of PCs were close between wild type and homozygous weaver. Similar data were found in the lobules of the lateral hemisphere. On the other hand, the loss of PCs in weaver cerebella can be related to foliar concavities or convexities depending on the vermal lobe or the hemispheric lobule studied. Lastly, we have obtained evidence that late-generated PCs of both normal and mutant mice were preferentially located in fissures. These quantitative relationships lead us to propose a model in which the final distribution of PCs through the vermal contour would be coupled to two factors: the cortical fissuration patterning and a "time-sequential effect" of weaver mutation.
为了确定浦肯野细胞（PCs）的生成与小脑沟回模式之间可能存在的关系，研究人员在织工小鼠的小脑中对浦肯野细胞的生成和定居情况进行了研究。在这些神经元产生时，给怀孕的雌性小鼠提供了氚标记的胸腺嘧啶核苷。然后，对出生后（P）90天的对照小鼠和织工小鼠后代的脑切片进行放射自显影。这使得根据位于两个叶区（裂隙和叶冠）的标记细胞比例，评估在不同胚胎时期产生的神经元的差异存活率成为可能。我们的数据表明，在整个蚓叶的表面轮廓上，野生型和纯合织工小鼠中浦肯野细胞的生成程序相近。在外侧半球的小叶中也发现了类似的数据。另一方面，织工小鼠小脑中浦肯野细胞的丢失可能与叶的凹陷或凸起有关，这取决于所研究的蚓叶或半球小叶。最后，我们获得的证据表明，正常小鼠和突变小鼠中晚期产生的浦肯野细胞都优先位于裂隙中。这些定量关系使我们提出一个模型，其中浦肯野细胞通过蚓叶轮廓的最终分布将与两个因素相关：皮质沟回模式和织工突变的“时间顺序效应”。
