通过逆转录酶-聚合酶链反应检测及对在巴西分离出的B亚型禽偏肺病毒进行分子特征分析

Detection by reverse transcriptase-polymerase chain reaction and molecular characterization of subtype B avian metapneumovirus isolated in Brazil.

作者信息

Chacón Jorge Luis, Brandão Paulo E, Buim Marcos, Villarreal Laura, Ferreira Antonio J Piantino

机构信息

Department of Pathology, University of São Paulo, Avenida Professor Doutor Orlando Marques de Paiva 87, Cidade Universitária, Butantã, São Paulo, SP, Brazil.

出版信息

Avian Pathol. 2007 Oct;36(5):383-7. doi: 10.1080/03079450701589142.

DOI:10.1080/03079450701589142

PMID:17899462

Abstract

Subtype B avian metapneumovirus (aMPV) was isolated and detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in Brazilian commercial laying chicken flocks with no history of vaccination against aMPV and presenting respiratory signs and decreased egg production. RT-PCR results from samples from three affected flocks revealed that the three isolates were subtype B. Partial sequence analysis of the G glycoprotein gene confirmed that the samples belonged to subtype B and were not of the vaccine type. Comparison of nucleotide and amino acid sequences of the G gene of the three Brazilian aMPV samples with subtype B isolates from other countries revealed 95.1% to 96.1% identity. Nucleotide sequences showed 100% identity among the Brazilian subtype B samples and 95.6% identity with the subtype B vaccine strain used in Brazil. This work describes the circulation of subtype B aMPV in Brazil and discusses its importance in terms of disease epidemiology.

摘要

在巴西未接种过禽偏肺病毒（aMPV）疫苗、出现呼吸道症状且产蛋量下降的商业蛋鸡群中，通过逆转录聚合酶链反应（RT-PCR）分离并检测到了B亚型禽偏肺病毒。来自三个受影响鸡群样本的RT-PCR结果显示，这三个分离株均为B亚型。G糖蛋白基因的部分序列分析证实，这些样本属于B亚型，而非疫苗株型。将三个巴西aMPV样本的G基因核苷酸和氨基酸序列与其他国家的B亚型分离株进行比较，发现同一性为95.1%至96.1%。核苷酸序列显示，巴西B亚型样本之间的同一性为100%，与巴西使用的B亚型疫苗株的同一性为95.6%。这项工作描述了B亚型aMPV在巴西的传播情况，并从疾病流行病学角度讨论了其重要性。