Lee Eun ho, Song Min-Suk, Shin Jin-Young, Lee Young-Min, Kim Chul-Joong, Lee Young Sik, Kim Hyunggee, Choi Young Ki
College of Medicine and Medical Research Institute, Chungbuk National University, 12 Gaeshin-Dong Heungduk-Ku, Cheongju 361-763, Republic of Korea.
Virus Res. 2007 Sep;128(1-2):18-25. doi: 10.1016/j.virusres.2007.03.029. Epub 2007 May 7.
Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in experimentally re-infected pheasants was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR). Chickens and mice were experimentally inoculated with PL-1 to test the replication potential of PL-1 in other species. Although one specimen from the nasal turbinates of an inoculated chicken showed a slight trace of viral replication at 3 days post-infection (dpi), all of the infected mice were negative for aMPV by RT-PCR throughout the experiment, suggesting that PL-1 does not readily infect mammals. This is the first report of the isolation and complete genomic sequence of aMPV subtype C originating from pheasants.
从雉鸡中分离出两种禽偏肺病毒（aMPV），分别命名为PL - 1和PL - 2，其完整核苷酸序列揭示了韩国首个全序列测定的aMPV的新序列。PL - 1和PL - 2的完整基因组均由13,170个核苷酸组成。系统发育分析表明，PL - 1属于aMPV C亚型，在推导的氨基酸序列同一性上与hMPV的同源性更高，而不是与aMPV A和B亚型。通过逆转录（RT）-聚合酶链反应（PCR）证实了PL - 1在实验性再感染雉鸡中的复制。对鸡和小鼠进行实验性接种PL - 1，以测试PL - 1在其他物种中的复制潜力。虽然接种鸡的鼻甲中的一个样本在感染后3天（dpi）显示出轻微的病毒复制痕迹，但在整个实验过程中，所有感染小鼠的aMPV RT - PCR检测均为阴性，这表明PL - 1不易感染哺乳动物。这是源自雉鸡的aMPV C亚型的分离和完整基因组序列的首次报道。
