Vinogradov Elena, He Huamei, Lubag Angelo, Balschi James A, Sherry A Dean, Lenkinski Robert E
Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
Magn Reson Med. 2007 Oct;58(4):650-5. doi: 10.1002/mrm.21393.
In this report, the On resonance PARamagnetic CHemical Exchange Effects (OPARACHEE) method was implemented in vivo using WALTZ-16* as a preparation pulse with a standard spin echo sequence to detect the accumulation and clearance of the TmDOTA-4AmC(-) in mouse kidney. The performance of the technique in vivo is described in terms of the magnitude of the contrast effect versus the bolus agent concentration and signal-to-noise ratio (SNR) levels. The lowest injected concentration of TmDOTA-4AmC(-), 200 microL of a 2-mM stock solution (corresponds to approximately 0.2 mM agent in plasma), reduced the total water signal in the kidney papilla by 45% 3 min after the a bolus injection. The results show that the OPARACHEE methodology employing low-amplitude RF trains can detect paramagnetic exchanging agents in vivo.
在本报告中，采用WALTZ - 16*作为制备脉冲，结合标准自旋回波序列，在体内实施了共振顺磁化学交换效应（OPARACHEE）方法，以检测TmDOTA - 4AmC(-)在小鼠肾脏中的蓄积和清除情况。该技术在体内的性能通过对比效应幅度与团注剂浓度以及信噪比（SNR）水平来描述。TmDOTA - 4AmC(-)的最低注射浓度，即2 mM储备溶液200微升（相当于血浆中约0.2 mM的试剂），在团注注射后3分钟使肾乳头中的总水信号降低了45%。结果表明，采用低幅度射频脉冲串的OPARACHEE方法能够在体内检测顺磁交换剂。
