使用化学交换饱和传递（CEST）MRI 进行肾脏 pH 值成像：基本概念。

Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST) MRI: Basic Concept.

机构信息

Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy.

University of Campania "Luigi Vanvitelli", Napoli, Italy.

出版信息

Methods Mol Biol. 2021;2216:241-256. doi: 10.1007/978-1-0716-0978-1_14.

DOI:10.1007/978-1-0716-0978-1_14

PMID:33476004

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9703214/

Abstract

Magnetic Resonance Imaging (MRI) has been actively explored in the last several decades for assessing renal function by providing several physiological information, including glomerular filtration rate, renal plasma flow, tissue oxygenation and water diffusion. Within MRI, the developing field of chemical exchange saturation transfer (CEST) has potential to provide further functional information for diagnosing kidney diseases. Both endogenous produced molecules as well as exogenously administered CEST agents have been exploited for providing functional information related to kidney diseases in preclinical studies. In particular, CEST MRI has been exploited for assessing the acid-base homeostasis in the kidney and for monitoring pH changes in several disease models. This review summarizes several CEST MRI procedures for assessing kidney functionality and pH, for monitoring renal pH changes in different kidney injury models and for evaluating renal allograft rejection.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This introduction chapter is complemented by two separate chapters describing the experimental procedure and data analysis.

摘要

磁共振成像（MRI）在过去几十年中一直被积极探索，通过提供包括肾小球滤过率、肾血浆流量、组织氧合和水扩散在内的几种生理信息来评估肾功能。在 MRI 中，化学交换饱和传递（CEST）这一新兴领域具有提供用于诊断肾脏疾病的进一步功能信息的潜力。内源性产生的分子以及外源性给予的 CEST 试剂都已被用于提供与临床前研究中肾脏疾病相关的功能信息。特别是，CEST MRI 已被用于评估肾脏中的酸碱平衡以及监测几种疾病模型中的 pH 值变化。这篇综述总结了几种用于评估肾脏功能和 pH 值的 CEST MRI 程序，用于监测不同肾脏损伤模型中的肾 pH 值变化，并评估肾移植排斥反应。这一章是基于 COST 行动 PARENCHIMA 的工作，该行动是一个由欧洲合作科学技术（COST）计划资助的、由社区驱动的网络，旨在提高肾脏 MRI 生物标志物的再现性和标准化。这一介绍性章节由两个单独的章节进行补充，描述了实验程序和数据分析。