Zhang Lei, Chen Huaiqing, Xiong Wang, Nguyen Tran
Institute of Biomedical Engineering, West China Center of Medical Scicences, Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2007 Aug;24(4):846-51.
Bone marrow mesenchymal stem cells (BMSCs) can be directed to differentiate into a variety of cell types depending on their micro-environment. In this study, rat BMSCs were co-cultured with rat ligament fibroblasts during different time courses. The mRNA expressions of type I, type III collagens and tenascin-C were measured by real time RT-PCR, and the corresponding protein levels of type I and type III collagens by radioimmunoassay. Results show that the mRNA expressions of type I and type III collagens in the BMSCs were 2 times up-regulated after a 6-day co-culture, and the relative mRNA expressions of type I and type III collagens were 3.9 +/- 0.2 and 1.9 +/- 0.2, while they were 1.9 +/- 0.3 and 0.8 +/- 0.1 in the control groups, respectively. The protein syntheses of these two collagens were also increased after a 12-day co-culture; the type I and type III collagens synthesis were 13.6 +/- 1.3 ng/microg and 5.9 +/- 0.5 ng/microg in co-culture groups and 12.4 +/- 0.8 ng/microg and 5.0 +/- 0.4 ng/microg in their control groups, respectively. Likewise, there was a 2 times enhancement in tenascin-C mRNA expression after the 12-day co-culture (0.07 +/- 0.02 by control group and 0.14 +/- 0.02 by co-culture group, P < 0. 05). These data suggest that the presence of the ligament fibroblast promotes the syntheses of type I and the III collagens and tenascin-C in the rat BMSC.
骨髓间充质干细胞（BMSCs）可根据其微环境被诱导分化为多种细胞类型。在本研究中，将大鼠BMSCs与大鼠韧带成纤维细胞在不同时间进程中进行共培养。通过实时逆转录聚合酶链反应（RT-PCR）检测I型、III型胶原蛋白和腱生蛋白-C的mRNA表达，并通过放射免疫测定法检测I型和III型胶原蛋白的相应蛋白水平。结果显示，共培养6天后，BMSCs中I型和III型胶原蛋白的mRNA表达上调了2倍，I型和III型胶原蛋白的相对mRNA表达分别为3.9±0.2和1.9±0.2，而对照组分别为1.9±0.3和0.8±0.1。共培养12天后，这两种胶原蛋白的蛋白质合成也增加；共培养组中I型和III型胶原蛋白的合成分别为13.6±1.3 ng/μg和5.9±0.5 ng/μg，对照组分别为12.4±0.8 ng/μg和5.0±0.4 ng/μg。同样，共培养12天后，腱生蛋白-C的mRNA表达增强了2倍（对照组为0.07±0.02，共培养组为0.14±0.02，P<0.05）。这些数据表明，韧带成纤维细胞的存在促进了大鼠BMSC中I型和III型胶原蛋白以及腱生蛋白-C的合成。
