Lukács Anita, Lengyel Zsuzsanna, Institóris L, Szabó Andrea
Department of Public Health, Faculty of Medicine, University of Szeged, Dom tér 10, H-6720 Szeged, Hungary.
Acta Biol Hung. 2007 Sep;58(3):259-67. doi: 10.1556/ABiol.58.2007.3.2.
Young adult male Wistar rats were treated, by gavage, with 80 or 320 mg/kg Pb2+ (lead acetate), 0.4 or 1.6 mg/kg Hg2+ (mercuric chloride) or both by combining the lower doses. For combination with alcohol, ethanol was added to the rats' drinking water in 5 v/v %. After 12 weeks of treatment, electrophysiological recording was made from the somatosensory cortex in urethane anaesthesia. Evoked potentials obtained by stimulation of the whiskers were recorded. Both metals, and alcohol alone, increased significantly the latency of the evoked response. Alcohol seemed to abolish the effect of Pb, but not of Hg. Fatigue, calculated form the response amplitude, was increased by Pb and Hg treatment and this effect of Hg was reduced by ethanol. Evoked activity and its dynamic characteristics were sensitive to the complex neurotoxic effect induced in the rats and can provide a basis for developing functional markers.
选用年轻成年雄性Wistar大鼠，通过灌胃给予80或320 mg/kg Pb2 +（醋酸铅）、0.4或1.6 mg/kg Hg2 +（氯化汞），或通过组合低剂量同时给予这两种金属。为了与酒精联合，将乙醇以5 v/v%的比例添加到大鼠的饮用水中。治疗12周后，在乌拉坦麻醉下从体感皮层进行电生理记录。记录通过刺激触须获得的诱发电位。两种金属以及单独的酒精均显著增加了诱发电反应的潜伏期。酒精似乎消除了铅的作用，但对汞没有作用。根据反应幅度计算的疲劳度在铅和汞处理后增加，而乙醇降低了汞的这种作用。诱发电活动及其动态特征对大鼠诱导的复合神经毒性作用敏感，可为开发功能标志物提供依据。
