Notermans S, Dufrenne J, in 't Veld P
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Int J Food Microbiol. 1991 Dec;14(3-4):325-31. doi: 10.1016/0168-1605(91)90124-8.
A reference material for staphylococcal enterotoxin A (SEA), was produced by spray-drying the toxin in milk. With this procedure the SEA was distributed homogeneously in the material. For ease of handling the reference material was encased in gelatin capsules, each containing 405 ng of SEA. Simply dissolving the milk powder in distilled water resulted in a 100% recovery of the SEA present. The reference material would appear suitable for testing laboratory performance, comparison of detection methods and to validation of extraction procedures.
一种用于葡萄球菌肠毒素A(SEA)的参考物质,是通过将毒素在牛奶中进行喷雾干燥制成的。通过此程序,SEA在该物质中均匀分布。为便于处理,参考物质被装入明胶胶囊中,每个胶囊含有405纳克SEA。只需将奶粉溶解于蒸馏水中,即可实现SEA的100%回收率。该参考物质似乎适用于测试实验室性能、比较检测方法以及验证提取程序。