使用夹心酶联免疫吸附测定法检测食物中毒暴发中食品中的葡萄球菌肠毒素A。
The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning.
作者信息
Wieneke A A, Gilbert R J
出版信息
J Hyg (Lond). 1985 Aug;95(1):131-8. doi: 10.1017/s0022172400062367.
Foods from outbreaks of food poisoning were examined for the presence of staphylococcal enterotoxin A (SEA) by a sandwich ELISA using microtitre trays as the solid phase and SEA antibodies raised in sheep. The presence of SEA was confirmed by neutralization tests. The toxin was detected in 12 of 15 foods from separate outbreaks of staphylococcal food poisoning; all 15 foods contained a strain of Staphylococcus aureus which produced SEA. For most foods a simple extraction procedure without a concentration step was sufficient to detect the toxin. The method was semi-quantitative and recoveries of SEA added to control foods varied from 30 to 80%. The foods from outbreaks contained between 1 and 10 micrograms of SEA/100 g. SEA was not found in foods from 21 outbreaks in which an SEA-producing strain of Staph. aureus was not isolated.
采用以微量滴定板为固相、用羊制备的抗葡萄球菌肠毒素A（SEA）抗体的夹心酶联免疫吸附测定法（ELISA），对食物中毒暴发事件中的食品进行检测，以确定其中是否存在SEA。通过中和试验确认SEA的存在。在15起单独的葡萄球菌食物中毒暴发事件中的12份食品中检测到了该毒素；所有15份食品均含有一株能产生SEA的金黄色葡萄球菌。对于大多数食品，无需浓缩步骤的简单提取程序就足以检测到毒素。该方法为半定量方法，添加到对照食品中的SEA回收率在30%至80%之间。暴发事件中的食品每100克含有1至10微克SEA。在21起未分离出能产生SEA的金黄色葡萄球菌菌株的食物中毒暴发事件的食品中未发现SEA。
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