博勒虹彩病毒作为异源基因表达的载体。

Bohle iridovirus as a vector for heterologous gene expression.

作者信息

Pallister Jackie, Goldie Sarah, Coupar Barbara, Shiell Brian, Michalski Wojtek P, Siddon Nicole, Hyatt Alex

机构信息

CSIRO Livestock Industries, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, Victoria 3220, Australia.

出版信息

J Virol Methods. 2007 Dec;146(1-2):419-23. doi: 10.1016/j.jviromet.2007.08.016. Epub 2007 Sep 29.

DOI:10.1016/j.jviromet.2007.08.016

PMID:17904650

Abstract

The large double-stranded DNA (ds DNA) viruses were among the first to be used to construct recombinant viruses, but to date this has not been achieved with any members of the ds DNA virus family, Iridoviridae. We identified a non-essential gene, the viral homologue of eukaryotic initiation factor 2alpha (eIF-2alpha), in Bohle iridovirus (BIV, genus Ranavirus). A recombinant BIV was constructed with the neomycin resistance gene and the Bufo marinus (cane toad) adult globin gene inserted into the BIV eIF-2alpha region. Adult globin expressed by the virus was detected on western blot, demonstrating that foreign genes can be expressed by the recombinant BIV in vitro and suggesting the possibility of using a recombinant BIV in the biological control of cane toads.

摘要

大型双链DNA（ds DNA）病毒是最早被用于构建重组病毒的病毒之一，但迄今为止，虹彩病毒科（Iridoviridae）这一双链DNA病毒家族的任何成员都尚未实现这一目标。我们在博勒虹彩病毒（BIV，蛙病毒属）中鉴定出一个非必需基因，即真核起始因子2α（eIF-2α）的病毒同源物。构建了一种重组BIV，将新霉素抗性基因和海蟾蜍成体珠蛋白基因插入到BIV的eIF-2α区域。通过蛋白质免疫印迹法检测到病毒表达的成体珠蛋白，这表明重组BIV能够在体外表达外源基因，并提示了使用重组BIV进行海蟾蜍生物防治的可能性。