Hunnicutt L E, Mozoruk J, Hunter W B, Crosslin J M, Cave R D, Powell C A
USDA ARS US Horticultural Research Laboratory, Ft. Pierce, FL 34945, USA.
Arch Virol. 2008;153(1):61-7. doi: 10.1007/s00705-007-1066-2. Epub 2007 Sep 28.
Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of this virus with a sensitivity of approximately 95 genome equivalents. A survey employing this assay in conjunction with GWSS samples collected throughout the United States including California, Hawaii, Florida Georgia, and the Carolinas revealed a fairly widespread pattern of distribution, although potentially restricted to temperate regions, areas with elevated host densities, or to populations of a common origin. The virus was found to naturally infect adults regardless of host plant and was not specific to a particular lifestage or sex. Examination of alternate leafhopper species further demonstrated that, although infection is not ubiquitous to all sharpshooter genera, HoCV-1 is not limited to Homalodisca vitripennis (=H. coagulata).
透射电子显微镜被用于确认在成年玻璃翅叶蝉（GWSS）中肠区域存在推测为葡萄叶蝉病毒-1（HoCV-1）的类微小核糖核酸病毒颗粒。此外，我们提供了一种逆转录聚合酶链反应（RT-PCR）检测方法来检测这种病毒，其灵敏度约为95个基因组当量。一项使用该检测方法并结合在美国各地（包括加利福尼亚州、夏威夷州、佛罗里达州、佐治亚州和卡罗来纳州）采集的GWSS样本的调查显示，尽管可能局限于温带地区、寄主密度较高的地区或共同起源的种群，但该病毒呈现出相当广泛的分布模式。发现该病毒能自然感染成年叶蝉，无论其寄主植物是什么，并且不特定于某个特定的生命阶段或性别。对其他叶蝉物种的检查进一步表明，虽然并非所有叶蝉属都会感染，但HoCV-1并不局限于葡萄叶蝉（=凝结叶蝉）。
