Banas Agnieszka, Yamamoto Yusuke, Teratani Takumi, Ochiya Takahiro
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan.
Dev Dyn. 2007 Dec;236(12):3228-41. doi: 10.1002/dvdy.21330.
Many studies on stem cell plasticity are challenging the concept that stem cells contain an intrinsically predefined, unidirectional differentiation program. This means that the developmental fate of a stem cell is dependent on the general potential of the cell (pre-determined stem cell fate) as well as on microenvironmental cues, such as stimuli from growth factors (stem cell niche). Here, we reviewed reports that examined the hepatocyte differentiation ability of stem cells from two different sources: embryonic stem cells and adult stem cells. All of those stem cells revealed the ability to give rise to hepatocyte-like cells using different induction strategies. However, it is still not clear which of those stem cells would be the best source for hepatocyte replacement or which would be the best protocol. We herein present the current knowledge regarding available protocols and factors used in order to obtain functional hepatocytes from stem cells.
许多关于干细胞可塑性的研究正在挑战干细胞包含内在预定义的单向分化程序这一概念。这意味着干细胞的发育命运既取决于细胞的一般潜能（预先确定的干细胞命运），也取决于微环境线索，如来自生长因子的刺激（干细胞龛）。在此，我们综述了研究两种不同来源干细胞（胚胎干细胞和成体干细胞）肝细胞分化能力的报告。所有这些干细胞都显示出使用不同诱导策略产生肝细胞样细胞的能力。然而，目前仍不清楚这些干细胞中哪一种是肝细胞替代的最佳来源，或者哪种是最佳方案。我们在此介绍关于从干细胞获得功能性肝细胞所使用的现有方案和因素的当前知识。
