Galer-Tatarowicz Katarzyna, Wiergowski Marek, Szpiech Beata, Reguła Krystyna, Jankowski Zbigniew
Z Katedry i Zakfadu Medycyny Sedowej Akademii Medycznej w Gdarisku Kierownik: dr hab. med. Z. Jankowski.
Arch Med Sadowej Kryminol. 2007 Jul-Sep;57(3):277-84.
The objective of the study was the presentation of the type and extent of drug addiction in the region covered by the Department of Forensic Medicine of the Medical University of Gdańsk based on the results of analyses of biological samples collected from the living and deceased subjects during the decade from 1996 to 2005. The studies were based on 110 cases of lethal intoxication after psychoactive substances overdosing and 63 cases of living people involved in the infringement of the law. Body-fluids (blood, urine) and post-mortem samples (stomach contents, small intestine contents, liver, kidney, brain and lung tissues) were collected and stored at -20 OC. The screening analysis of urine was performed using the Fluorescence Polarization Immunoassay method (TDx, Abbott) and the enzyme immunoassay method (Syva Rapid Test, Dade Behring). The confirmation of positive results was carried out by thin-layer chromatography (TLC), as well as gas and liquid chromatography with various detectors (GC/FID, GC/NPD, GC/MS, LC/UV-DAD). In the last 10 years, there was noted a 3-fold increase in the number of cases that required an analysis of narcotics content, and in the last 4 years, such cases exceeded 10% of the total number of investigated cases. The number of analyses aiming at determination of amphetamine and cannabinol contents in living people (mainly among individuals that caused traffic accidents) increased. Among deceased subjects, deaths were predominantly caused by intoxication with opiates or combinations of opiates and other narcotic substances and medications. The presence of both ethanol and drugs was detected and confirmed mainly in post-mortem biological materials.
本研究的目的是根据1996年至2005年这十年间从活体和死者身上采集的生物样本分析结果，呈现格但斯克医科大学法医学系所覆盖地区药物成瘾的类型和程度。研究基于110例精神活性物质过量致死中毒案例和63例涉及违法的活体案例。采集了体液（血液、尿液）和死后样本（胃内容物、小肠内容物、肝脏、肾脏、脑和肺组织）并储存在-20℃。尿液的筛查分析采用荧光偏振免疫分析法（TDx，雅培公司）和酶免疫分析法（Syva快速检测，达德拜林公司）。阳性结果通过薄层色谱法（TLC）以及配备各种检测器的气相和液相色谱法（GC/FID、GC/NPD、GC/MS、LC/UV-DAD）进行确认。在过去十年中，需要分析麻醉品含量的案例数量增加了3倍，在过去四年中，此类案例超过了调查案例总数的10%。针对活体（主要是交通事故肇事者）中苯丙胺和大麻酚含量测定的分析数量增加。在死者中，死亡主要由阿片类药物或阿片类药物与其他麻醉物质及药物的组合中毒引起。乙醇和药物的存在主要在死后生物材料中被检测和确认。
