Chavatte-Palmer P, Guillomot M, Roïz J, Heyman Y, Laigre P, Servely J L, Constant F, Hue I, Ellis S A
INRA, UMR 1198, ENVA, CNRS, FRE 2857, Biologie du Développement et Reproduction, Jouy en Josas, France.
Cloning Stem Cells. 2007 Fall;9(3):346-56. doi: 10.1089/clo.2006.0086.
Abnormally increased placental expression of major histocompatibility complex class I (MHC-I) molecules at the trophoblastic surface has been suggested previously to be the cause of early fetal loss in nuclear transfer (NT) bovine pregnancies. Here, we report the lack of expression of MHC-I at the trophoblastic surface at D30 and D60 and in placentomes from D60 to term in placentas obtained by NT from three different genotypes and by artificial insemination, whatever the outcome of the pregnancy. MHC-I expression was assessed by immunohistochemistry using four different antibodies, including a novel beta2-microglobulin antibody. The MHC-I type of the clones was established using reference strand-mediated conformation analysis (RSCA); however, since it proved problematic to type the recipient animals in the same way, outcome of pregnancy could not be related to MHC compatibility. In conclusion, the present study provides no evidence to support abnormal expression of MHC-I on the trophoblastic surface in clones as a major cause of fetal loss during pregnancy after NT.
先前有研究表明，滋养层表面主要组织相容性复合体I类（MHC-I）分子的胎盘表达异常增加是核移植（NT）牛妊娠早期胎儿丢失的原因。在此，我们报告，无论妊娠结果如何，通过NT从三种不同基因型以及通过人工授精获得的胎盘在第30天和第60天的滋养层表面以及从第60天到足月的胎盘小叶中均未检测到MHC-I的表达。使用四种不同的抗体（包括一种新型β2-微球蛋白抗体）通过免疫组织化学评估MHC-I的表达。使用参考链介导的构象分析（RSCA）确定克隆的MHC-I类型；然而，由于以同样的方式对受体动物进行分型存在问题，因此妊娠结果与MHC相容性无关。总之，本研究没有提供证据支持克隆中滋养层表面MHC-I的异常表达是NT后妊娠期间胎儿丢失的主要原因。
